Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

článek v časopise ve Web of Science, Jimp

angličtina

Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.

Bovine serum albumin; Constant-current chronopotentiometric stripping; Electrodeposition; Protein; Silver amalgam particles

Sebest, P.; Ostatna, V.; Fojta, M.; Danhel, A.

15. 2. 2020

ELSEVIER SCIENCE SA

LAUSANNE

1572-6657

J ELECTROANAL CHEM

859

1

Švýcarská konfederace

113854-1

113854-5

5

https://doi.org/10.1016/j.jelechem.2020.113854