Detail publikace

Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation

HASHEMI, A. EZATI, M. MOHAMMADNEJAD, J. HOUSHMAND, B. FAGHIHI, S.

Originální název

Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background: Ineffective integration has been recognized as one of the major causes of early orthopedic failure of titanium-based implants. One strategy to address this problem is to develop modified titanium surfaces that promote osteoblast differentiation. This study explored titanium surfaces modified with TiO2 nanotubes (TiO2 NTs) capable of localized drug delivery into bone and enhanced osteoblast cell differentiation. Materials and Methods: Briefly, TiO2 NTs were subjected to anodic oxidation and loaded with Metformin, a widely used diabetes drug. To create surfaces with sustainable drug-eluting characteristics, TiO2 NTs were spin coated with a thin layer of chitosan. The surfaces were characterized via scanning electron microscopy, atomic force microscopy, and contact angle measurements. The surfaces were then exposed to mesenchymal bone marrow stem cells (MSCs) to evaluate cell adhesion, growth, differentiation, and morphology on the modified surfaces. Results: A noticeable increase in drug release time (3 days vs 20 days) and a decrease in burst release characteristics (85% to 7%) was observed in coated samples as compared to uncoated samples, respectively. Chitosan-coated TiO2 NTs exhibited a considerable enhancement in cell adhesion, proliferation, and genetic expression of type I collagen, and alkaline phosphatase activity as compared to uncoated TiO2 NTs. Conclusion: TiO2 NT surfaces with a chitosan coating are capable of delivering Metformin to a bone site over a sustained period of time with the potential to enhance MSCs cell attachment, proliferation, and differentiation.

Klíčová slova

titania nanotubes; titanium; osteogenic differentiation; anodization; mesenchymal bone marrow stem cells; MSCs

Autoři

HASHEMI, A.; EZATI, M.; MOHAMMADNEJAD, J.; HOUSHMAND, B.; FAGHIHI, S.

Vydáno

22. 6. 2020

Nakladatel

DOVE MEDICAL PRESS LTD

Místo

ALBANY

ISSN

1178-2013

Periodikum

International Journal of Nanomedicine

Ročník

15

Číslo

1

Stát

Nový Zéland

Strany od

4471

Strany do

4481

Strany počet

11

URL

https://www.dovepress.com/chitosan-coating-of-tio2-nanotube-arrays-for-improved-metformin-releas-peer-reviewed-fulltext-article-IJN

BibTex

@article{BUT171654,
  author="Amir {Hashemi} and Masoumeh {Ezati} and Javad {Mohammadnejad} and Behzad {Houshmand} and Shahab {Faghihi}",
  title="Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation",
  journal="International Journal of Nanomedicine",
  year="2020",
  volume="15",
  number="1",
  pages="4471--4481",
  doi="10.2147/IJN.S248927",
  issn="1178-2013",
  url="https://www.dovepress.com/chitosan-coating-of-tio2-nanotube-arrays-for-improved-metformin-releas-peer-reviewed-fulltext-article-IJN"
}