Detail publikace

The Effect of Bio-Conditioning of Titanium Implants for Enhancing Osteogenic Activity

MONTAZERI, M. HASHEMI, A. HOUSHMAND, B. FAGHIHI, S.

Originální název

The Effect of Bio-Conditioning of Titanium Implants for Enhancing Osteogenic Activity

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Early and effective integration of titanium-based materials into bone tissue is of vital importance for long-term stability of implants. Surface modification is commonly used to enhance cell-substrate interactions for improving cell adhesion, proliferation, and activity. Here, the surface of titanium substrates and commercial implants were coated with blood (TiB), fetal bovine serum (TiF), and phosphate-buffered saline (TiP) solution using a spin coating process. Surface roughness and wettability of samples were measured using contact angle measurements and atomic force microscopy. The samples were then exposed to human osteoblast-like MG63 cells in order to evaluate adhesion, growth, differentiation, and morphology on the surface of modified samples. Untreated titanium disks were used as controls. The lowest roughness and wettability values were found in unmodified titanium samples followed by TiP, TiF, and TiB. The percentage of cellular attachment and proliferation for each sample was measured using an MTT (3-[4,5-dimethylthiazol-2yl] 2,5diphenyl-2H-tetrazoliumbromide) assay. Cell adhesion and proliferation were most improved on TiB followed closely by TiF. The results of this study revealed an increased expression of the osteogenic marker protein alkaline phosphatase on TiB and the coated commercial titanium implants. These results suggested that precoating titanium samples with blood may improve cellular response by successfully mimicking a physiological environment that could be beneficial for clinical implant procedures.

Klíčová slova

biological modification; titanium; ossteoinduction; osteoblast-like MG63 cells; dental implant

Autoři

MONTAZERI, M.; HASHEMI, A.; HOUSHMAND, B.; FAGHIHI, S.

Vydáno

1. 6. 2019

Nakladatel

ALLEN PRESS INC

Místo

LAWRENCE

ISSN

0160-6972

Periodikum

The Journal of oral implantology

Ročník

45

Číslo

3

Stát

Spojené státy americké

Strany od

187

Strany do

195

Strany počet

9

URL

https://meridian.allenpress.com/joi/article-abstract/45/3/187/365042/The-Effect-of-Bio-Conditioning-of-Titanium?redirectedFrom=fulltext

BibTex

@article{BUT171655,
  author="Mohadeseh {Montazeri} and Amir {Hashemi} and Behzad {Houshmand} and Shahab {Faghihi}",
  title="The Effect of Bio-Conditioning of Titanium Implants for Enhancing Osteogenic Activity",
  journal="The Journal of oral implantology",
  year="2019",
  volume="45",
  number="3",
  pages="187--195",
  doi="10.1563/aaid-joi-D-18-00020",
  issn="0160-6972",
  url="https://meridian.allenpress.com/joi/article-abstract/45/3/187/365042/The-Effect-of-Bio-Conditioning-of-Titanium?redirectedFrom=fulltext"
}