Detail publikace

Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

ZEMÁNKOVÁ, K. PAVELICOVÁ, K. BEZDĚKOVÁ, J. NEJDL, L. VEJVODOVÁ, M. VACULOVIČ, T. VACULOVIČOVÁ, M.

Originální název

Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

In this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICP-MS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.

Klíčová slova

Metallothionein, Molecularly imprinted polymers, Magnetic particles, LA-ICP-MS

Autoři

ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; BEZDĚKOVÁ, J.; NEJDL, L.; VEJVODOVÁ, M.; VACULOVIČ, T.; VACULOVIČOVÁ, M.

Vydáno

12. 7. 2021

Nakladatel

Tanger

ISBN

978-80-87294-98-7

Kniha

NANOCON 2020 CONFERENCE PROCEEDINGS

Edice

1st

ISSN

2694-930X

Periodikum

Proceedings 12th International Conference on Nanomaterials - Research & Application

Stát

Česká republika

Strany od

385

Strany do

389

Strany počet

5

URL

https://www.confer.cz/nanocon/2020/3749-mettalothionein-sensing-using-molecularly-imprinted-polymers-coupled-to-laser-ablation-inductively-coupled-plasma-mass-spectrometry

Plný text v Digitální knihovně

http://hdl.handle.net/11012/200490

BibTex

@inproceedings{BUT172000,
  author="Kristýna {Zemánková} and Kristýna {Pavelicová} and Jaroslava {Bezděková} and Lukáš {Nejdl} and Markéta {Vejvodová} and Tomáš {Vaculovič} and Markéta {Vaculovičová}",
  title="Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry",
  booktitle="NANOCON 2020 CONFERENCE PROCEEDINGS",
  year="2021",
  series="1st",
  journal="Proceedings 12th International Conference on Nanomaterials - Research & Application",
  pages="385--389",
  publisher="Tanger",
  doi="10.37904/nanocon.2020.3749",
  isbn="978-80-87294-98-7",
  issn="2694-930X",
  url="https://www.confer.cz/nanocon/2020/3749-mettalothionein-sensing-using-molecularly-imprinted-polymers-coupled-to-laser-ablation-inductively-coupled-plasma-mass-spectrometry"
}