Detail publikace

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

SKOPALÍK, J. SEKORA, J. PEŠL, M. BÉBAROVÁ, M. ŠVECOVÁ, O. PARÁK, T. ČMIEL, V. PROVAZNÍK, I. JEKLOVÁ, E. MAŠEK, J.

Originální název

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

Typ

článek v časopise ve Scopus, Jsc

Jazyk

angličtina

Originální abstrakt

Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.

Klíčová slova

isolated heart; heart perfusion; tissue thermostability; thermo controller; IR thermometry

Autoři

SKOPALÍK, J.; SEKORA, J.; PEŠL, M.; BÉBAROVÁ, M.; ŠVECOVÁ, O.; PARÁK, T.; ČMIEL, V.; PROVAZNÍK, I.; JEKLOVÁ, E.; MAŠEK, J.

Vydáno

1. 3. 2021

Nakladatel

Czech Society for Biomedical Engineering and Medical Informatics

Místo

Prague, Czech Republic

ISSN

0301-5491

Periodikum

Lékař a technika

Ročník

50

Číslo

4

Stát

Česká republika

Strany od

137

Strany do

141

Strany počet

5

URL

https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203210

BibTex

@article{BUT173201,
  author="Josef {Skopalík} and Jiří {Sekora} and Martin {Pešl} and Markéta {Bébarová} and Olga {Švecová} and Tomáš {Parák} and Vratislav {Čmiel} and Valentine {Provazník} and Edita {Jeklová} and Josef {Mašek}",
  title="Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater",
  journal="Lékař a technika",
  year="2021",
  volume="50",
  number="4",
  pages="137--141",
  doi="10.14311/CTJ.2020.4.03",
  issn="0301-5491",
  url="https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126"
}