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GAŇOVÁ, M. ZHANG, H. ZHU, H. KORABEČNÁ, M. NEUŽIL, P.
Originální název
Multiplexed digital polymerase chain reaction as a powerful diagnostic tool
Typ
článek v časopise ve Scopus, Jsc
Jazyk
angličtina
Originální abstrakt
The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced. In this review, we introduce dPCR techniques based on sample compartmentalization, droplet- and chip-based systems, and their combinations. We then discuss dPCR multiplexing methods in both laboratory research settings and advanced or routine clinical applications. We focus on their strengths and weaknesses with regard to the character of biological samples and to the required precision of such analysis, as well as showing recently published work based on those methods. Finally, we envisage possible future achievements in this field.
Klíčová slova
Digital polymerase chain reaction; Absolute quantification; Multiplexing strategies; Duplex multiplexing; Higher order multiplexing; Clinical applications
Autoři
GAŇOVÁ, M.; ZHANG, H.; ZHU, H.; KORABEČNÁ, M.; NEUŽIL, P.
Vydáno
1. 6. 2021
Nakladatel
Elsevier
Místo
Amsterdam
ISSN
1873-4235
Periodikum
BIOSENSORS & BIOELECTRONICS
Ročník
181
Číslo
1
Stát
Nizozemsko
Strany od
113155-1
Strany do
113155-12
Strany počet
12
URL
https://www.sciencedirect.com/science/article/pii/S0956566321001925?dgcid=rss_sd_all