Experimental evaluation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) applicability in biomedicine

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Bioplastics are recently presumed as a convenient solution of the world pollution. In the foreground, group of bacterial biopolymers – polyhyd-roxyalkanoates, tends to satisfy several demands placed on these materials. Amongst PHAs, copolymer of 3-hydroxybutyrate and 4-hydroxy-butyrate – P(3HB-co-4HB) presents promising properties for wide range of applications. This potential bioplastic is naturally accumulated by a thermophilic strain Aneurinibacillus sp. H1. Since this producent is a Gram-positive bacterium, it does not produce endotoxins and is willing to generate adjustable monomer-ratio polymer according to cultivation conditions.In this study, we present crucial biophysical properties of copolymer P(3HB-co-4HB) manufactured by employing Aneurinibacillus sp. H1, in order to evaluate its applicability in biomedicine. Two different-ratio copolymers were produced, as a referential material, homopolymer pol-y-3-hydroxybutyrate was manufactured by cultivating mesophilic bacterium Cupriavidius necator H16. Surface characterisation of polymers films was performed by scanning electron microscopy and atomic force microscopy. To evaluate active ingredients release from the materials, dye diffusion tests with spectroscopic detection were provided. Surface electro optics was employed to determine contact angle of wettability. Finally, in vitro biodegradation tests in simulated body fluids were held, the rate of degradation had been observed by molar mass and mass decreases determination and microscopic surface observation.

Poly-3-hydroxybutyrate-co-4-hydroxybutyrate, Aneurinibacillus sp. H1, biomedical applications

VODIČKA, J.; WIKARSKÁ, M.; POSPÍŠILOVÁ, A.; SLANINOVÁ, E.; TRUDIČOVÁ, M.; MENČÍK, P.; OBRUČA, S.; SEDLÁČEK, P.

1. 12. 2021