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MALINA, T. POLÁKOVÁ, K. SKOPALÍK, J. MILOTOVÁ, V. HOLÁ, K. HAVRDOVÁ, M. TOMÁNKOVÁ, K. ČMIEL, V. ŠEFC, L. ZBOŘIL, R.
Originální název
Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells (vol 152, pg 434, 2019)
Typ
různé
Jazyk
angličtina
Originální abstrakt
Tissue regeneration based on stem cell therapy is one of the most rapidly developing fields of modern medicine. Several properties of human mesenchymal stromal cells (MSCs), such as tropism toward a tumor or injury site, make them promising candidates for regenerative medicine, targeted therapy, or treating injured tissues. However, to fully understand the role of stem cells in therapeutic function, their visualization in vivo is essential. Here, we describe, for the first time, the use of biocompatible quaternized carbon dots (QCDs) as a novel stem-cell tracking probe for in vivo fluorescence imaging of transplanted human MSCs. By studying the in vitro cytotoxicity, intracellular distribution, and precise uptake mechanism, we showed that QCDs had a high biocompatibility and excellent fluorescence properties after 24 h incubation with MSCs. Further to demonstrate the in vivo feasibility of the system, QCD-labeled MSCs (100 mu g/mL of QCDs, 24 h incubation time) were transplanted subcutaneously into an immunodeficient mouse and visualized by optical in vivo imaging. The labeled cells were strongly fluorescent, allowing their semi-quantitative detection. Moreover, the homing of intravenously transplanted QCD-labeled MSCs into the solid tumor was clearly shown. The results demonstrated that QCD-labeling of human MSCs is a highly promising approach for in vivo tracking during stem cell therapy. (C) 2019 Elsevier Ltd. All rights reserved.
Klíčová slova
Carbon dots; Stem cell tracking; Cytotoxicity; In vitro and in vivo optical imaging; Photoluminescence
Autoři
MALINA, T.; POLÁKOVÁ, K.; SKOPALÍK, J.; MILOTOVÁ, V.; HOLÁ, K.; HAVRDOVÁ, M.; TOMÁNKOVÁ, K.; ČMIEL, V.; ŠEFC, L.; ZBOŘIL, R.
Vydáno
15. 4. 2021
Nakladatel
PERGAMON-ELSEVIER SCIENCE LTD
Místo
OXFORD
ISSN
0008-6223
Periodikum
CARBON
Ročník
174
Stát
Spojené státy americké
Strany od
771
Strany do
Strany počet
1
BibTex
@misc{BUT177159, author="Tomáš {Malina} and Kateřina {Poláková} and Josef {Skopalík} and Věra {Milotová} and Kateřina {Holá} and Markéta {Havrdová} and Kateřina {Tománková} and Vratislav {Čmiel} and Luděk {Šefc} and Radek {Zbořil}", title="Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells (vol 152, pg 434, 2019)", year="2021", journal="CARBON", volume="174", pages="771--771", publisher="PERGAMON-ELSEVIER SCIENCE LTD", address="OXFORD", doi="10.1016/j.carbon.2020.11.028", issn="0008-6223", note="miscellaneous" }