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VODIČKA, J. WIKARSKÁ, M. TRUDIČOVÁ, M. JUGLOVÁ, Z. POSPÍŠILOVÁ, A. KALINA, M. SLANINOVÁ, E. OBRUČA, S. SEDLÁČEK, P.
Originální název
Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate-P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.
Klíčová slova
polyhydroxyalkanoates (PHA); Aneurinibacillus sp. H1; PHA copolymers; biodegradation; simulated body fluids
Autoři
VODIČKA, J.; WIKARSKÁ, M.; TRUDIČOVÁ, M.; JUGLOVÁ, Z.; POSPÍŠILOVÁ, A.; KALINA, M.; SLANINOVÁ, E.; OBRUČA, S.; SEDLÁČEK, P.
Vydáno
13. 5. 2022
Nakladatel
MDPI
Místo
BASEL
ISSN
2073-4360
Periodikum
Polymers
Ročník
14
Číslo
10
Stát
Švýcarská konfederace
Strany od
1
Strany do
Strany počet
URL
https://www.mdpi.com/2073-4360/14/10/1990
Plný text v Digitální knihovně
http://hdl.handle.net/11012/204434
BibTex
@article{BUT177660, author="Juraj {Vodička} and Monika {Wikarská} and Monika {Trudičová} and Zuzana {Juglová} and Aneta {Pospíšilová} and Michal {Kalina} and Eva {Slaninová} and Stanislav {Obruča} and Petr {Sedláček}", title="Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids", journal="Polymers", year="2022", volume="14", number="10", pages="1--14", doi="10.3390/polym14101990", issn="2073-4360", url="https://www.mdpi.com/2073-4360/14/10/1990" }