Detail publikace

Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases

ZÁMOCKÝ, M. MUSIL, M. BANCHENKO, M. FERIANC, P. CHOVANOVÁ, K. BARÁTH, P. POLJOVKA, A. BEDNÁŘ, D.

Originální název

Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, we focus on a detailed bioinformatics analysis of hyBpox genes, mainly within the genomes of Sclerotiniaceae (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or biotrophic host specialists. Members of the genus Sclerotium produce only sclerotia and no fruiting bodies or spores. Thus, their physiological role for peroxidases remains open. A representative species, S. cepivorum, is a dangerous plant pathogen causing white rot in Allium species, particularly in onions, leeks, and garlic. On a worldwide basis, the white rot caused by this soil-borne fungus is apparently the most serious threat to Allium-crop production. We have also found very similar peroxidase sequences in the related fungus S. sclerotiorum, although with minor yet important modifications in the architecture of its active centre. The presence of ScephyBpox1-specific mRNA was confirmed by transcriptomic analysis. The presence of Hybrid B peroxidase at the protein level as the sole extracellular peroxidase of this fungus was confirmed in the secretome of S. cepivorum through detailed proteomic analyses. This prompted us to systematically search for all available genes coding for Hybrid B heme peroxidases in the whole fungal family of Sclerotiniaceae. We present here a reconstruction of their molecular phylogeny and analyse the unique aspects of their conserved-sequence features and structural folds in corresponding ancestral sequences.

Klíčová slova

hybrid B heme peroxidase, peroxidasecatalase superfamily, oxidative stress, enzymatic antioxidant, ancestral sequence reconstruction

Autoři

ZÁMOCKÝ, M.; MUSIL, M.; BANCHENKO, M.; FERIANC, P.; CHOVANOVÁ, K.; BARÁTH, P.; POLJOVKA, A.; BEDNÁŘ, D.

Vydáno

17. 3. 2022

ISSN

2079-7737

Periodikum

Biology

Ročník

11

Číslo

3

Stát

Švýcarská konfederace

Strany od

1

Strany do

18

Strany počet

18

URL

https://www.mdpi.com/2079-7737/11/3/459

BibTex

@article{BUT177747,
  author="Marcel {Zámocký} and Miloš {Musil} and Maksym {Banchenko} and Peter {Ferianc} and Katarína {Chovanová} and Peter {Baráth} and Andrej {Poljovka} and David {Bednář}",
  title="Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases",
  journal="Biology",
  year="2022",
  volume="11",
  number="3",
  pages="1--18",
  doi="10.3390/biology11030459",
  issn="2079-7737",
  url="https://www.mdpi.com/2079-7737/11/3/459"
}