Detail publikace

Use of MALDI-TOF mass spectrometry for virus identification: a review

DO, T. GURÁŇ, R. ADAM, V. ZÍTKA, O.

Originální název

Use of MALDI-TOF mass spectrometry for virus identification: a review

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) directly or indirectly affects every individual worldwide. The fight against SARS-CoV-2 is based on the rapid and accurate diagnosis and subsequent isolation of infected individuals. Therefore, the demands for the scientific development of diagnostic methods for the confirmation of SARS-CoV-2 are enormous. Currently, reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is the main method used for detecting viruses, including SARS-CoV-2, and is considered the gold standard for coronavirus disease 2019 (COVID-19) identification. However, various alternatives have been investigated due to the time and cost demands of this method or to shortages of reagents. In this review, we focus on matrix-assisted laser desorption and ionisation with time-of-flight analyser mass spectrometry (MALDI-TOF MS) techniques as potential tools for the diagnosis of viruses with an emphasis on SARS-CoV-2. MALDI-TOF is commonly used in clinical laboratories for bacterial characterization and identification, but in the field of clinical virology, MALDI-TOF remains only a promising technology for routine diagnosis. This review provides an overview of the development of clinical virology from the point of view of using MALDI-TOF for virus identification and as a possible diagnostic tool for SARS-CoV-2 detection. In addition, this review summarizes the current state of standard methods for virus diagnostics including the preparation of clinical samples.

Klíčová slova

MALDI-TOF; mass spectrometry; virus; identification; SARS-CoV-2

Autoři

DO, T.; GURÁŇ, R.; ADAM, V.; ZÍTKA, O.

Vydáno

15. 6. 2022

ISSN

0003-2654

Periodikum

ANALYST

Ročník

147

Číslo

14

Stát

Spojené království Velké Británie a Severního Irska

Strany od

3131

Strany do

3154

Strany počet

24

URL

https://doi.org/10.1039/D2AN00431C

BibTex

@article{BUT178395,
  author="Tomáš {Do} and Roman {Guráň} and Vojtěch {Adam} and Ondřej {Zítka}",
  title="Use of MALDI-TOF mass spectrometry for virus identification: a review",
  journal="ANALYST",
  year="2022",
  volume="147",
  number="14",
  pages="3131--3154",
  doi="10.1039/d2an00431c",
  issn="0003-2654",
  url="https://doi.org/10.1039/D2AN00431C"
}