Detail publikace

Addressing cancer invasion and cell motility with quantitative light microscopy

ZICHA, D.

Originální název

Addressing cancer invasion and cell motility with quantitative light microscopy

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The incidence of death caused by cancer has been increasing worldwide. The growth of cancer cells is not the main problem. The majority of deaths are due to invasion and metastasis, where cancer cells actively spread from primary tumors. Our inbred rat model of spontaneous metastasis revealed dynamic phenotype changes in vitro correlating with the metastatic potential in vivo and led to a discovery of a metastasis suppressor, protein 4.1B, which affects their 2D motility on flat substrates. Subsequently, others confirmed 4.1B as metastasis suppressor using knock-out mice and patient data suggesting mechanism involving apoptosis. There is evidence that 2D motility may be differentially controlled to the 3D situation. Here we show that 4.1B affects cell motility in an invasion assay similarly to the 2D system, further supporting our original hypothesis that the role of 4.1B as metastasis suppressor is primarily mediated by its effect on motility. This is encouraging for the validity of the 2D analysis, and we propose Quantitative Phase Imaging with incoherent light source for rapid and accurate testing of cancer cell motility and growth to be of interest for personalized cancer treatment as illustrated in experiments measuring responses of human adenocarcinoma cells to selected chemotherapeutic drugs.

Klíčová slova

Quantitative phase imaging, cancer cell behavior

Autoři

ZICHA, D.

Vydáno

10. 1. 2022

Nakladatel

NATURE PORTFOLIO

Místo

BERLIN

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

12

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1621

Strany do

1621

Strany počet

11

URL

https://www.nature.com/articles/s41598-022-05307-7

Plný text v Digitální knihovně

http://hdl.handle.net/11012/208734

BibTex

@article{BUT178800,
  author="Daniel {Zicha}",
  title="Addressing cancer invasion and cell motility with quantitative light microscopy",
  journal="Scientific Reports",
  year="2022",
  volume="12",
  number="1",
  pages="1621--1621",
  doi="10.1038/s41598-022-05307-7",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-022-05307-7"
}