Detail publikace

Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure

HYMPANOVA, M. OLIVER-URRUTIA, C. VOJTA, M. MACHÁČEK, M. KŘUPKA, P. KUKLA, R. ČELKO, L. MONTUFAR, E. B. MAREK, J.

Originální název

Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Streptococcus mutans is one of the bacteria that initiates the colonization of the pellicle at the tooth surface. It forms a plaque, together with other bacteria, which gradually dissolves the pellicle and leaves the tooth surface unprotected against the acidic oral environment. Calcium phosphate ceramics are excellent synthetic materials for the study of biofilm formation in dentistry because they are comparable to teeth in chemical composition and structure. Calcium phosphates can be processed to achieve a variety of crystalline compounds with biologically relevant ionic substitutions and structures that allow study of the effect of the surface chemistry and the topography independently. In this article, we describe the preparation and characterization of three types of calcium phosphate-based materials as a suitable surface for the formation of the S. mutans biofilm: beta-tricalcium phosphate (beta-TCP); sintered hydroxyapatite (SHA); and calcium-deficient hydroxyapatite (CDHA). The densest biofilms were formed on the surfaces of SHA and CDHA, with no significant differences due to the stoichiometry or microstructure. In contrast, beta-TCP showed a lower susceptibility to S. mutans biofilm formation, suggesting that the crystalline structure is the controlling parameter. Subsequently, SHA was selected to develop a dental biofilm model that allowed study of S. mutans biofilm susceptibility to chlorhexidine and ethanol.

Klíčová slova

Calcium phosphate; Oral biofilm model; Streptococcus mutans; Biofilm; Bacteria

Autoři

HYMPANOVA, M.; OLIVER-URRUTIA, C.; VOJTA, M.; MACHÁČEK, M.; KŘUPKA, P.; KUKLA, R.; ČELKO, L.; MONTUFAR, E. B.; MAREK, J.

Vydáno

18. 3. 2022

Nakladatel

ELSEVIER

Místo

AMSTERDAM, NETHERLANDS

ISSN

2772-9508

Periodikum

BIOMATERIALS ADVANCES

Ročník

135

Číslo

212750

Stát

Nizozemsko

Strany počet

8

URL

https://www.sciencedirect.com/science/article/pii/S2772950822000279

BibTex

@article{BUT179190,
  author="HYMPANOVA, M. and OLIVER-URRUTIA, C. and VOJTA, M. and MACHÁČEK, M. and KŘUPKA, P. and KUKLA, R. and ČELKO, L. and MONTUFAR, E. B. and MAREK, J.",
  title="Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure",
  journal="BIOMATERIALS ADVANCES",
  year="2022",
  volume="135",
  number="212750",
  pages="8",
  doi="10.1016/j.bioadv.2022.212750",
  issn="2772-9508",
  url="https://www.sciencedirect.com/science/article/pii/S2772950822000279"
}