Detail publikace

Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis

DUFKOVÁ, K. BEZDÍČEK, M. NYKRÝNOVÁ, M. KOCMANOVÁ, I. KUBÁČKOVÁ, P. HANSLIKOVA, J. FEJKOVÁ, K. MAYER, J. LENGEROVÁ, M.

Originální název

Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The Pseudomonas aeruginosa population has a nonclonal epidemic structure. It is generally composed of a limited number of widespread clones selected from a background of many rare and unrelated genotypes recombining at high frequency. Due to the increasing prevalence of nosocomial infections caused by multidrug-resistant/extensively drug-resistant (MDR/XDR) strains, it is advisable to implement infection control measures. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are considered the gold standard methods in bacterial typing, despite being limited by cost, staff, and instrumental demands. Here, we present a novel mini-MLST scheme for P. aeruginosa rapid genotyping based on high-resolution melting analysis. Using the proposed mini-MLST scheme, 3,955 existing sequence types (STs) were converted into 701 melting types (MelTs), resulting in a discriminatory power of D = 0.993 (95% confidence interval [CI], 0.992 to 0.994). Whole-genome sequencing of 18 clinical isolates was performed to support the newly designed mini-MLST scheme. The clonal analysis of STs belonging to MelTs associated with international high-risk clones (HRCs) performed by goeBURST software revealed that a high proportion of the included STs are highly related to HRCs and have also been witnessed as responsible for serious infections. Therefore, mini-MLST provides a clear warning for the potential spread of P. aeruginosa clones recognized as MDR/XDR strains with possible serious outcomes.

Klíčová slova

Pseudomonas aeruginosa; high-resolution melting; mini-MLST; molecular epidemiology; strain typing

Autoři

DUFKOVÁ, K.; BEZDÍČEK, M.; NYKRÝNOVÁ, M.; KOCMANOVÁ, I.; KUBÁČKOVÁ, P.; HANSLIKOVA, J.; FEJKOVÁ, K.; MAYER, J.; LENGEROVÁ, M.

Vydáno

11. 1. 2023

Nakladatel

American Society for Microbiology

ISSN

2165-0497

Periodikum

Microbiology spectrum

Ročník

11

Číslo

1

Stát

Spojené státy americké

Strany od

1

Strany do

9

Strany počet

9

URL

https://journals.asm.org/doi/10.1128/spectrum.03571-22

Plný text v Digitální knihovně

http://hdl.handle.net/11012/209214

BibTex

@article{BUT180802,
  author="Kristýna {Dufková} and Matěj {Bezdíček} and Markéta {Jakubíčková} and Iva {Kocmanová} and Petra {Kubáčková} and Jana {Hanslikova} and Karolína {Fejková} and Jiří {Mayer} and Martina {Lengerová}",
  title="Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis",
  journal="Microbiology spectrum",
  year="2023",
  volume="11",
  number="1",
  pages="1--9",
  doi="10.1128/spectrum.03571-22",
  issn="2165-0497",
  url="https://journals.asm.org/doi/10.1128/spectrum.03571-22"
}