Detail publikace

An innovative biodevice towards monitoring of miR-153 using specific DNA immobilized on the surface of poly(chitosan) decorated AgNPrs/GQDs-CysA conductive nano-ink: Early-stage diagnosis of Parkinson's disease using biosensor technology

Aminabad, ED. Hasanzadeh, M. Saadati, A. Feizi, MAH. Safaralizadeh, R. Mobed, A.

Originální název

An innovative biodevice towards monitoring of miR-153 using specific DNA immobilized on the surface of poly(chitosan) decorated AgNPrs/GQDs-CysA conductive nano-ink: Early-stage diagnosis of Parkinson's disease using biosensor technology

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Parkinson's disease (PD), as the world's second most common disabling age-related disease has caused motor and non-motor symptoms such as mental and behavioral changes, sleep problems, depression, memory difficulties. In recent years, several advantages of microRNAs (miRNAs), including resistance to RNAase digestion, stability, and extreme situations in circulating fluids, have led to received enormous attention for the detection of various diseases. miR-153 is one of the high potential related biomarkers of PDs which regulate alpha-Synuclein expression levels. Here, a sensitive and selective electrochemical biosensor was developed to recognize of miR-153 in human plasma samples. For the first time, the surface of the glassy carbon electrode (GCE) was decorated with polychitosan (CS) and silver nanoprisms/graphene quantum dots/ cysteamine A (AgNPrs/GQDs-Cys A) ink for the excellent immobilization of DNA towards sensitive and selective monitoring of micRNA-153. The designed novel electrochemical biosensor could detect miR-153 by differential pulse voltammetry with a linear range of 6.25 mu M to 50 mu M, which low limit of quantification is 6.25 mu M. According to high selectivity, sensitivity, fast and easy operation this biodevice promising for clinical applications and high-throughput screening of PDs.

Klíčová slova

Parkinson; Electrochemical biosensor; miR-153; Nano-ink; Biological macromolecule; DNA; Biomedical analysis

Autoři

Aminabad, ED.; Hasanzadeh, M.; Saadati, A.; Feizi, MAH.; Safaralizadeh, R.; Mobed, A.

Vydáno

1. 12. 2022

Nakladatel

ELSEVIER

Místo

AMSTERDAM

ISSN

1873-4944

Periodikum

Materials Science and Engineering B-Advanced Functional Solid-State Materials

Ročník

286

Číslo

116017

Stát

Nizozemsko

Strany počet

9

URL

https://www.sciencedirect.com/science/article/pii/S0921510722004056?via%3Dihub

BibTex

@article{BUT182261,
  author="Aminabad, ED. and Hasanzadeh, M. and Saadati, A. and Feizi, MAH. and Safaralizadeh, R. and Mobed, A.",
  title="An innovative biodevice towards monitoring of miR-153 using specific DNA immobilized on the surface of poly(chitosan) decorated AgNPrs/GQDs-CysA conductive nano-ink: Early-stage diagnosis of Parkinson's disease using biosensor technology",
  journal="Materials Science and Engineering B-Advanced Functional Solid-State Materials",
  year="2022",
  volume="286",
  number="116017",
  pages="9",
  doi="10.1016/j.mseb.2022.116017",
  issn="1873-4944",
  url="https://www.sciencedirect.com/science/article/pii/S0921510722004056?via%3Dihub"
}