Detail publikace

Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia

MATIAŠOVIC, J. BZDIL, J. PAPEŽÍKOVÁ, I. ČEJKOVÁ, D. VASINA, E. BIZOS, J. NAVRÁTIL, S. ŠEDIVÁ, M. KLAUDINY, J. PIKULA, J.

Originální název

Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybee larvae. In the Czech Republic, two large infested regions were recognised. This study aimed to analyse P. larvae strains occurring in the Czech Republic in the years 2016–2017 and to characterise the genetic structure of their population with the use of Enterobacterial Repetitive Intergenic Consensus genotyping (ERIC), multilocus sequence typing (MLST) and whole genome sequence (WGS) analysis. The results were complemented by the analysis of isolates collected in the year 2018 in areas of Slovakia located near the Czechia-Slovakia border. ERIC genotyping revealed that 78.9% of tested isolates belonged to the ERIC II genotype and 21.1% to ERIC I genotype. MLST showed six sequence types with ST10 and ST11 being the most frequent among isolates. Within six isolates we found discrepancies in correlations between MLST and ERIC genotypes. The use of MLST and WGS analysis of isolates revealed that each of the large infested geographic regions had its own dominating P. larvae strains. We assume that these strains represented primary sources of infection in the affected areas. In addition, the sporadic presence of strains identified by core genome analysis as genetically related was unveiled in geographically distant regions suggesting possible human-mediated transmission of AFB.

Klíčová slova

American foulbroodGenotypingEpidemiologyHoneybeeAFB spread

Autoři

MATIAŠOVIC, J.; BZDIL, J.; PAPEŽÍKOVÁ, I.; ČEJKOVÁ, D.; VASINA, E.; BIZOS, J.; NAVRÁTIL, S.; ŠEDIVÁ, M.; KLAUDINY, J.; PIKULA, J.

Vydáno

8. 3. 2023

Nakladatel

ELSEVIER

ISSN

1532-2661

Periodikum

RESEARCH IN VETERINARY SCIENCE

Ročník

158

Číslo

3

Stát

Spojené království Velké Británie a Severního Irska

Strany od

34

Strany do

40

Strany počet

7

URL

https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_

BibTex

@article{BUT183134,
  author="Ján {Matiašovic} and Jaroslav {Bzdil} and Ivana {Papežíková} and Darina {Čejková} and Evgeniya {Vasina} and Jiří {Bizos} and Stanislav {Navrátil} and Mária {Šedivá} and Jaroslav {Klaudiny} and Jiří {Pikula}",
  title="Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia",
  journal="RESEARCH IN VETERINARY SCIENCE",
  year="2023",
  volume="158",
  number="3",
  pages="34--40",
  doi="10.1016/j.rvsc.2023.03.007",
  issn="1532-2661",
  url="https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_"
}