Detail publikace

Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks

NYARUABA, R. MWALIKO, C. DOBNIK, D. NEUZIL, P. AMOTH, P. MWAU, M. YU, J. YANG, H. WEI, H.P.

Originální název

Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health disaster. The current gold standard for the diagnosis of infected patients is real-time reverse transcription-quantitative PCR (RT-qPCR). As effective as this method may be, it is subject to false-negative and -positive results, affecting its precision, especially for the detection of low viral loads in samples. In contrast, digital PCR (dPCR), the third generation of PCR, has been shown to be more effective than the gold standard, RT-qPCR, in detecting low viral loads in samples. In this review article, we selected publications to show the broad-spectrum applications of dPCR, including the development of assays and reference standards, environmental monitoring, mutation detection, and clinical diagnosis of SARS-CoV-2, while comparing it analytically to the gold standard, RT-qPCR. In summary, it is evident that the specificity, sensitivity, reproducibility, and detection limits of RT-dPCR are generally unaffected by common factors that may affect RT-qPCR. As this is the first time that dPCR is being tested in an outbreak of such a magnitude, knowledge of its applications will help chart a course for future diagnosis and monitoring of infectious disease outbreaks.

Klíčová slova

RT-dPCR; RT-qPCR; SARS-CoV-2; COVID-19; ddPCR; diagnosis; quantification; viral load

Autoři

NYARUABA, R.; MWALIKO, C.; DOBNIK, D.; NEUZIL, P.; AMOTH, P.; MWAU, M.; YU, J.; YANG, H.; WEI, H.P.

Vydáno

21. 9. 2022

Nakladatel

American Society for Microbiology

Místo

USA

ISSN

1098-6618

Periodikum

Clinical Microbiology Reviews

Ročník

35

Číslo

3

Stát

Spojené státy americké

Strany počet

37

URL

https://journals.asm.org/doi/full/10.1128/cmr.00168-21

BibTex

@article{BUT183674,
  author="NYARUABA, R. and MWALIKO, C. and DOBNIK, D. and NEUZIL, P. and AMOTH, P. and MWAU, M. and YU, J. and YANG, H. and WEI, H.P.",
  title="Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks
",
  journal="Clinical Microbiology Reviews",
  year="2022",
  volume="35",
  number="3",
  pages="37",
  doi="10.1128/cmr.00168-21",
  issn="1098-6618",
  url="https://journals.asm.org/doi/full/10.1128/cmr.00168-21"
}