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DRÁPALOVÁ, E. MICHLOVSKÁ, L. POŠTULKOVÁ, H. CHAMRADOVÁ, I. LIPOVÝ, B. HOLOUBEK, J. VACEK, L. RŮŽIČKA, F. HANSLIANOVÁ, M. SVOBODOVÁ, T. ČERNÁ, E. HRDLIČKOVÁ, B. VOJTOVÁ, L.
Originální název
Antimicrobial Cost-Effective Transparent Hydrogel Films from Renewable Gum Karaya/Chitosan Polysaccharides for Modern Wound Dressings
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The newest trends in wound healing management and the development of the next generation of dressings are pointing toward natural polymeric materials with important beneficial properties such as antimicrobial effects, renewability, easier process of preparation, and biological activity. Here, we present the preparation and in vitro evaluation of a unique biopolymeric blend composed of natural polymers based on the positively charged polysaccharide chitosan and negatively charged gum karaya. A plate lysis assay of gum karaya and chitosan solution mixtures proved the synergistic antimicrobial effect against specific strains of both Gram-positive and Gram-negative bacteria and yeast. This polymeric mixture was used for hydrogel film preparation and determination of the composition effect on physical properties (swelling behavior in different solvents, pH, diffusion mechanism, hydrolytic stability, mechanical and optical properties). While the pure gum karaya with poly(vinyl alcohol) exhibited the highest hydrolytic degradation (68%), the mixture of poly(vinyl alcohol) and gum karaya with chitosan (in the 25:75 ratio) exhibited the lowest degradation value (41%) due to the strong physical interactions. Cytotoxicity tests performed with hydrogel extracts using two different in vitro models, adherent fibroblasts (NIH3T3) and non-adherent suspension B-lymphocytes (BaF3), exhibited excellent biocompatibility and no cytotoxicity. As expected, the antimicrobial activity of 3-day film extracts showed a significantly improved antimicrobial effect of mixtures involving a chitosan biopolymer. The physical and biological properties of prepared biopolymer-based hydrogels meet the requirements of modern wound dressings.
Klíčová slova
natural gum; polysaccharides; biopolymers; antibacterial activity; cytotoxicity
Autoři
DRÁPALOVÁ, E.; MICHLOVSKÁ, L.; POŠTULKOVÁ, H.; CHAMRADOVÁ, I.; LIPOVÝ, B.; HOLOUBEK, J.; VACEK, L.; RŮŽIČKA, F.; HANSLIANOVÁ, M.; SVOBODOVÁ, T.; ČERNÁ, E.; HRDLIČKOVÁ, B.; VOJTOVÁ, L.
Vydáno
14. 4. 2023
Nakladatel
American Chemical Society
Místo
WASHINGTON
ISSN
2637-6105
Periodikum
ACS APPLIED POLYMER MATERIALS
Ročník
5
Číslo
4
Stát
Spojené státy americké
Strany od
2774
Strany do
2786
Strany počet
13
URL
https://pubs.acs.org/doi/10.1021/acsapm.3c00025
Plný text v Digitální knihovně
http://hdl.handle.net/11012/213684
BibTex
@article{BUT183757, author="Eva {Drápalová} and Lenka {Michlovská} and Hana {Poštulková} and Ivana {Chamradová} and Břetislav {Lipový} and Jakub {Holoubek} and Lukáš {Vacek} and Filip {Růžička} and Markéta {Hanslianová} and Táňa {Svobodová} and Eva {Černá} and Barbara {Hrdličková} and Lucy {Vojtová}", title="Antimicrobial Cost-Effective Transparent Hydrogel Films from Renewable Gum Karaya/Chitosan Polysaccharides for Modern Wound Dressings", journal="ACS APPLIED POLYMER MATERIALS", year="2023", volume="5", number="4", pages="2774--2786", doi="10.1021/acsapm.3c00025", issn="2637-6105", url="https://pubs.acs.org/doi/10.1021/acsapm.3c00025" }