Detail publikace

Time-lapse Quantitative Phase Imaging for the Study of Cell Migration

KŘÍŽOVÁ, A. NOVÁKOVÁ, Z. ŠURÁŇOVÁ, M.

Originální název

Time-lapse Quantitative Phase Imaging for the Study of Cell Migration

Typ

audiovizuální tvorba

Jazyk

angličtina

Originální abstrakt

Quantitative Phase Imaging (QPI) is a powerful label-free technique widely used to study the dynamic processes of living cells in real-time. QPI in time-lapse microscopy offers several benefits, including high sensitivity, low phototoxicity, high spatial and temporal resolution, and quantitative measurements of cellular morphology and mechanical properties. In this presentation, we will discuss using of QPI to study cell migration, a fundamental biological process that is critical for tissue development and repair. We will demonstrate how QPI can provide detailed insights into the dynamics of cell migration, including the speed of cell movement, the direction of migration, and the evaluation of the migrastatic drugs, or the way of migration in extracellular matrices. Additionally, we will show how QPI can be used to quantify cellular parameters such as cell dry mass, area, perimeter, migration index, or Euclidean distance. Our results demonstrate the potential of QPI in time-lapse microscopy as a powerful tool for studying cell migration and other dynamic processes in living cells.

Klíčová slova

microscopy, time-lapse, cell migration

Autoři

KŘÍŽOVÁ, A.; NOVÁKOVÁ, Z.; ŠURÁŇOVÁ, M.

Vydáno

5. 6. 2023

Strany počet

95

BibTex

@misc{BUT183848,
  author="Aneta {Křížová} and Zuzana {Nováková} and Markéta {Šuráňová}",
  title="Time-lapse Quantitative Phase Imaging for the Study of Cell Migration",
  booktitle="VÝROČNÍ KONFERENCE ČSMS, MIKROSKOPIE 2023",
  year="2023",
  pages="95",
  note="presentation"
}