Detail publikace

Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots

ASSI, N. RÝPAR, T. MACKA, M. ADAM, V. VACULOVIČOVÁ, M.

Originální název

Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Microfluidic paper-based analytical devices modified with molecularly imprinted polymers (mu PADs@MIPs) were developed for fluorescent detection of targeted thiols via in situ UV-induced formation of quantum dots (mu PADs@MIPs@QDs). The selectivity enhancement by the MIP layer formed on the filter paper surface was demonstrated for the isolation of L-homocysteine from wine. Followed by the addition of metal precursors solution (Zn/Cd/Cu) and UV irradiation, fluorescent quantum dots were formed thus enabling quantitative detection of the thiol (serving as a QD capping agent). The effect of different semiconductors was investigated to achieve a lower band gap and higher fluorescence intensity. Increasing fluorescence intensity in the presence of thiol groups was obtained for the following precursors mixture composition: ZnCdCu/S > ZnCd/S > ZnCu/S > ZnS. The proposed method has a good relationship between the fluorescence intensity of ZnCdCu/S QDs and L-homocysteine in a linear range from 0.74 to 7.40 mu M with a limit of detection (LOD) and quantification (LOQ) of 0.51 and 1.71 mu M respectively. This method was applied for the determination of L-homocysteine in white wine with RSD under 6.37%.

Klíčová slova

Thiol; Microfluidic paper-based analytical devices; Molecularly imprinted polymers; Quantum dots; White wine

Autoři

ASSI, N.; RÝPAR, T.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M.

Vydáno

1. 4. 2023

Nakladatel

ELSEVIER

Místo

AMSTERDAM

ISSN

1873-3573

Periodikum

TALANTA

Ročník

255

Číslo

124185

Stát

Spojené království Velké Británie a Severního Irska

Strany počet

7

URL

https://www.sciencedirect.com/science/article/pii/S003991402200981X?via%3Dihub

BibTex

@article{BUT183971,
  author="Navid {Assi} and Tomáš {Rýpar} and Miroslav {Macka} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots",
  journal="TALANTA",
  year="2023",
  volume="255",
  number="124185",
  pages="7",
  doi="10.1016/j.talanta.2022.124185",
  issn="1873-3573",
  url="https://www.sciencedirect.com/science/article/pii/S003991402200981X?via%3Dihub"
}