Detail publikace

Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

FIALOVÁ, L. ŽYLKOVÁ, K. MÁROVÁ, I.

Originální název

Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.

Klíčová slova

PCR, DNA isolation, polysaccharides, calcium chloride, mango

Autoři

FIALOVÁ, L.; ŽYLKOVÁ, K.; MÁROVÁ, I.

Vydáno

23. 10. 2023

Nakladatel

Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic

Místo

Brno

ISBN

978-80-908154-0-7

Kniha

CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis

Edice

1

Číslo edice

1

Strany od

50

Strany do

50

Strany počet

1

URL

https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

BibTex

@misc{BUT185429,
  author="Lenka {Fialová} and Kateřina {Žylková} and Ivana {Márová}",
  title="Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from
Tropical Fruit Products",
  booktitle="CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis",
  year="2023",
  series="1",
  edition="1",
  pages="50--50",
  publisher="Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic",
  address="Brno",
  isbn="978-80-908154-0-7",
  url="https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf",
  note="abstract"
}