Detail publikace

Genosensing on a 3D-printed nanocarbon electrode

JYOTI, J. FOJTA, M. HERMANOVÁ, M. PIVOŇKOVÁ, H. ALDUHAISH, O. PUMERA, M.

Originální název

Genosensing on a 3D-printed nanocarbon electrode

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.

Klíčová slova

3D-printed nanocarbon electrode; DNA hybridization; Electrochemical analysis; Additive manufacturing

Autoři

JYOTI, J.; FOJTA, M.; HERMANOVÁ, M.; PIVOŇKOVÁ, H.; ALDUHAISH, O.; PUMERA, M.

Vydáno

1. 6. 2023

Nakladatel

Elsevier

Místo

NEW YORK

ISSN

1873-1902

Periodikum

ELECTROCHEMISTRY COMMUNICATIONS

Ročník

151

Číslo

1

Stát

Spojené státy americké

Strany počet

8

URL

https://www.sciencedirect.com/science/article/pii/S1388248123000826

Plný text v Digitální knihovně

http://hdl.handle.net/11012/244975

BibTex

@article{BUT186966,
  author="Jyoti {Jyoti} and Miroslav {Fojta} and Monika {Hermanová} and Hana {Pivoňková} and Osamah {Alduhaish} and Martin {Pumera}",
  title="Genosensing on a 3D-printed nanocarbon electrode",
  journal="ELECTROCHEMISTRY COMMUNICATIONS",
  year="2023",
  volume="151",
  number="1",
  pages="8",
  doi="10.1016/j.elecom.2023.107508",
  issn="1873-1902",
  url="https://www.sciencedirect.com/science/article/pii/S1388248123000826"
}