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DZURENDOVÁ, S. OLSEN, P. BYRTUSOVÁ, D. TAFINTSEVA, V. SHAPAVAL, V. HORN, S. KOHLER, A. SZOTKOWSKI, M. MÁROVÁ, I. ZIMMERMANN, B.
Originální název
Raman spectroscopy online monitoring of biomass production, intracellular metabolites and carbon substrates during submerged fermentation of oleaginous and carotenogenic microorganisms
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Background: Monitoring and control of both growth media and microbial biomass is extremely important for the development of economical bioprocesses. Unfortunately, process monitoring is still dependent on a limited number of standard parameters (pH, temperature, gasses etc.), while the critical process parameters, such as biomass, product and substrate concentrations, are rarely assessable in-line. Bioprocess optimization and monitoring will greatly benefit from advanced spectroscopy-based sensors that enable real-time monitoring and control. Here, Fourier transform (FT) Raman spectroscopy measurement via flow cell in a recirculatory loop, in combination with predictive data modeling, was assessed as a fast, low-cost, and highly sensitive process analytical technology (PAT) system for online monitoring of critical process parameters. To show the general applicability of the method, submerged fermentation was monitored using two different oleaginous and carotenogenic microorganisms grown on two different carbon substrates: glucose fermentation by yeast Rhodotorula toruloides and glycerol fermentation by marine thraustochytrid Schizochytrium sp. Additionally, the online FT-Raman spectroscopy approach was compared with two at-line spectroscopic methods, namely FT-Raman and FT-infrared spectroscopies in high throughput screening (HTS) setups. Results: The system can provide real-time concentration data on carbon substrate (glucose and glycerol) utilization, and production of biomass, carotenoid pigments, and lipids (triglycerides and free fatty acids). Robust multivariate regression models were developed and showed high level of correlation between the online FT-Raman spectral data and reference measurements, with coefficients of determination (R-2) in the 0.94-0.99 and 0.89-0.99 range for all concentration parameters of Rhodotorula and Schizochytrium fermentation, respectively. The online FT-Raman spectroscopy approach was superior to the at-line methods since the obtained information was more comprehensive, timely and provided more precise concentration profiles. Conclusions: The FT-Raman spectroscopy system with a flow measurement cell in a recirculatory loop, in combination with prediction models, can simultaneously provide real-time concentration data on carbon substrate utilization, and production of biomass, carotenoid pigments, and lipids. This data enables monitoring of dynamic behaviour of oleaginous and carotenogenic microorganisms, and thus can provide critical process parameters for process optimization and control. Overall, this study demonstrated the feasibility of using FT-Raman spectroscopy for online monitoring of fermentation processes.
Klíčová slova
Process analytical technology; Raman spectroscopy; Infrared spectroscopy; Real-time monitoring; Partial least squares (PLS) regression; Lipids; Carotenoids; Rhodotorula; Schizochytrium
Autoři
DZURENDOVÁ, S.; OLSEN, P.; BYRTUSOVÁ, D.; TAFINTSEVA, V.; SHAPAVAL, V.; HORN, S.; KOHLER, A.; SZOTKOWSKI, M.; MÁROVÁ, I.; ZIMMERMANN, B.
Vydáno
18. 12. 2023
Nakladatel
BMC
Místo
LONDON
ISSN
1475-2859
Periodikum
Microbial Cell Factories
Ročník
22
Číslo
261
Stát
Spojené království Velké Británie a Severního Irska
Strany počet
14
URL
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02268-y
Plný text v Digitální knihovně
http://hdl.handle.net/11012/245209
BibTex
@article{BUT187228, author="Simona {Dzurendová} and Pernille Margrethe {Olsen} and Dana {Byrtusová} and Valeria {Tafintseva} and Volha {Shapaval} and Svein Jarle {Horn} and Achim {Kohler} and Martin {Szotkowski} and Ivana {Márová} and Boris {Zimmermann}", title="Raman spectroscopy online monitoring of biomass production, intracellular metabolites and carbon substrates during submerged fermentation of oleaginous and carotenogenic microorganisms", journal="Microbial Cell Factories", year="2023", volume="22", number="261", pages="14", doi="10.1186/s12934-023-02268-y", issn="1475-2859", url="https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02268-y" }