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HRUBANOVÁ, K. SIKOROVÁ, P. MRÁZOVÁ, K. NEBESÁŘOVÁ, J. OBRUČA, S. KRZYŽÁNEK, V.
Originální název
Morphological Study of PHA Producing Bacteria
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .
Klíčová slova
polyhydroxyalkanoates; electron microscopy; Cupriavidus necator
Autoři
HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.
Vydáno
31. 10. 2023
Nakladatel
Oxford Academic
Místo
Oxford
ISSN
1435-8115
Periodikum
Microscopy and Microanalysis
Ročník
29
Číslo
1
Stát
Spojené státy americké
Strany od
883
Strany do
884
Strany počet
2
URL
https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF
BibTex
@inproceedings{BUT187572, author="Kamila {Hrubanová} and Pavlína {Sikorová} and Kateřina {Mrázová} and Jana {Nebesářová} and Stanislav {Obruča} and Vladislav {Krzyžánek}", title="Morphological Study of PHA Producing Bacteria", booktitle="Mictoscopy and Microanalysis", year="2023", journal="Microscopy and Microanalysis", volume="29", number="1", pages="883--884", publisher="Oxford Academic", address="Oxford", doi="10.1093/micmic/ozad067.436", issn="1435-8115", url="https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF" }