Detail publikace

DNA Quadruplex Structure with a Unique Cation Dependency

GAJARSKÝ, M. STADLBAUER, P. CUCCHIARINI, A. ŠPONER, J. DOBROVOLNÁ, M. BRÁZDA, V. MERGNY, J. TRANTÍREK, L. LENARCIC ZIVKOVIC, M.

Originální název

DNA Quadruplex Structure with a Unique Cation Dependency

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

DNA quadruplex structures provide an additional layer of regulatory control in genome maintenance and gene expression and are widely used in nanotechnology. We report the discovery of an unprecedented tetrastranded structure formed from a native G-rich DNA sequence originating from the telomeric region of Caenorhabditis elegans. The structure is defined by multiple properties that distinguish it from all other known DNA quadruplexes. Most notably, the formation of a stable so-called KNa-quadruplex (KNaQ) requires concurrent coordination of K+ and Na+ ions at two distinct binding sites. This structure provides novel insight into G-rich DNA folding under ionic conditions relevant to eukaryotic cell physiology and the structural evolution of telomeric DNA. It highlights the differences between the structural organization of human and nematode telomeric DNA, which should be considered when using C. elegans as a model in telomere biology, particularly in drug screening applications. Additionally, the absence/presence of KNaQ motifs in the host/parasite introduces an intriguing possibility of exploiting the KNaQ fold as a plausible antiparasitic drug target. The structure's unique shape and ion dependency and the possibility of controlling its folding by using low-molecular-weight ligands can be used for the design or discovery of novel recognition DNA elements and sensors.

Klíčová slova

DNA; quadruplex; unique cation dependency; NMR spectroscopy; telomere

Autoři

GAJARSKÝ, M.; STADLBAUER, P.; CUCCHIARINI, A.; ŠPONER, J.; DOBROVOLNÁ, M.; BRÁZDA, V.; MERGNY, J.; TRANTÍREK, L. LENARCIC ZIVKOVIC, M.

Vydáno

24. 12. 2023

Nakladatel

WILEY-V C H VERLAG GMBH

Místo

WEINHEIM

ISSN

1433-7851

Periodikum

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Ročník

63

Číslo

7

Stát

Spolková republika Německo

Strany počet

11

URL

https://onlinelibrary.wiley.com/doi/10.1002/anie.202313226

BibTex

@article{BUT187729,
  author="GAJARSKÝ, M. and STADLBAUER, P. and CUCCHIARINI, A. and ŠPONER, J. and DOBROVOLNÁ, M. and BRÁZDA, V. and MERGNY, J. and TRANTÍREK, L.  LENARCIC ZIVKOVIC, M.",
  title="DNA Quadruplex Structure with a Unique Cation Dependency",
  journal="ANGEWANDTE CHEMIE-INTERNATIONAL EDITION",
  year="2023",
  volume="63",
  number="7",
  pages="11",
  doi="10.1002/anie.202313226",
  issn="1433-7851",
  url="https://onlinelibrary.wiley.com/doi/10.1002/anie.202313226"
}