Detail publikace

Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes

PAVLIŇÁKOVÁ, V. IZSÁK, D. LIPOVÝ, B. KNOZ, M. SMOLNICKÁ, A. FALDYNA, M. JEKLOVÁ, E. KOUTNÁ, I. VOJTOVÁ, L.

Originální název

Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes

Anglický název

Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes

Typ

abstrakt

Jazyk

čeština

Originální abstrakt

The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.

Anglický abstrakt

The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.

Klíčová slova

bilayer scaffold, skin, nanofibers, skin substitutes

Klíčová slova v angličtině

bilayer scaffold, skin, nanofibers, skin substitutes

Autoři

PAVLIŇÁKOVÁ, V.; IZSÁK, D.; LIPOVÝ, B.; KNOZ, M.; SMOLNICKÁ, A.; FALDYNA, M.; JEKLOVÁ, E.; KOUTNÁ, I.; VOJTOVÁ, L.

Vydáno

25. 4. 2024

Místo

Praha

ISBN

978-80-01-07293-6

Kniha

Bioimplantologie a regenerativní medicína 2024

Číslo edice

1. elektronické

Strany od

43

Strany do

43

Strany počet

59

URL

https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d

BibTex

@misc{BUT188633,
  author="Veronika {Pavliňáková} and Dávid {Izsák} and Břetislav {Lipový} and Martin {Knoz} and Anna {Smolnická} and Martin {Faldyna} and Edita {Jeklová} and Irena {Koutná} and Lucy {Vojtová}",
  title="Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes",
  booktitle="Bioimplantologie a regenerativní medicína 2024",
  year="2024",
  edition="1. elektronické",
  pages="43--43",
  address="Praha",
  isbn="978-80-01-07293-6",
  url="https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d",
  note="abstract"
}