Detail publikace

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

KOŠELOVÁ, Z. FOHLEROVÁ, Z.

Originální název

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

This research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of different-sized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.

Klíčová slova

gold nanoparticles, biosensors, enzyme immobilization, impedance spectroscopy, glucose detection, calibration curves

Autoři

KOŠELOVÁ, Z.; FOHLEROVÁ, Z.

Vydáno

23. 4. 2024

Nakladatel

Brno University of Technology, Faculty of Electrical Engineering

Místo

Brno

ISBN

978-80-214-6230-4

Kniha

Proceedings II of the 30 th Conference STUDENT EEICT 2024

Edice

1

ISSN

2788-1334

Periodikum

Proceedings II of the Conference STUDENT EEICT

Ročník

30

Číslo

1

Stát

Česká republika

Strany od

202

Strany do

206

Strany počet

5

URL

https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf

BibTex

@inproceedings{BUT189256,
  author="Zuzana {Košelová} and Zdenka {Fohlerová}",
  title="Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles",
  booktitle="Proceedings II of the 30 th Conference STUDENT EEICT 2024",
  year="2024",
  series="1",
  journal="Proceedings II of the Conference STUDENT EEICT",
  volume="30",
  number="1",
  pages="202--206",
  publisher="Brno University of Technology, Faculty of Electrical Engineering",
  address="Brno",
  isbn="978-80-214-6230-4",
  issn="2788-1334",
  url="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf"
}