Detail publikace

The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

MELČOVÁ, V. KROBOT, Š. ŠINDELÁŘ, J. ŠEBOVÁ, E. KLUSÁČEK RAMPICHOVÁ, M. PŘIKRYL, R.

Originální název

The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The aim of this work is to explore the effect of surface characteristics of the 3D printed scaffolds on their suitability for Saos-2 cells growth. Two promising materials for bone tissue engineering were examined, both containing poly(3-hydroxybutyrate)/poly(d,l-lactide) plasticized blend and one of them filled with bioactive tricalcium phosphate. The surface free energy (SFE) of samples was determined by contact angle measurement with four liquids. For non-filled sample the water contact angle (WCA) was 74° and its SFE was 40 mN∙m−1. The composite sample exhibited substantially decreased WCA, 61°. Moreover, the addition of the tricalcium phosphate caused doubling of the polar component of the SFE which increased by a total of 13%. 3D printed surfaces prepared by fused deposition modeling method showed a profound increase of WCA due to the increase in their surface roughness which was analyzed by confocal microscopy. The change in wetting properties strongly affected the behavior of Saos-2 cells on printed surfaces. The number of cells as measured by DNA quantification linearly decreased with increasing surface WCA. The highest number of cells was observed on the pressed samples and 3D printed surfaces made of simple lines and a grid with 50 μm gap between lines. The favored surfaces exhibit WCA under 80° which is important information for the future design of the scaffolds.

Klíčová slova

Poly(3-hydroxybutyrate); polylactide; 3D printing; water contact angle; surface energy; surface roughness

Autoři

MELČOVÁ, V.; KROBOT, Š.; ŠINDELÁŘ, J.; ŠEBOVÁ, E.; KLUSÁČEK RAMPICHOVÁ, M.; PŘIKRYL, R.

Vydáno

1. 8. 2024

Nakladatel

Elsevier

ISSN

2666-8459

Periodikum

Results in Surfaces and Interfaces

Ročník

16

Číslo

August

Stát

Nizozemsko

Strany od

1

Strany do

18

Strany počet

18

URL

https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub

BibTex

@article{BUT189307,
  author="Veronika {Melčová} and Štěpán {Krobot} and Jan {Šindelář} and Eva {Šebová} and Michala {Klusáček Rampichová} and Radek {Přikryl}",
  title="The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces",
  journal="Results in Surfaces and Interfaces",
  year="2024",
  volume="16",
  number="August",
  pages="1--18",
  doi="10.1016/j.rsurfi.2024.100271",
  issn="2666-8459",
  url="https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub"
}