Detail publikace

Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

PRANADA, A. ČIČATKA, M. KARÁSEK, J. HEß, C.

Originální název

Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.

Klíčová slova

agar plates, synthetic image generation, deep learning, semantic segmentation

Autoři

PRANADA, A.; ČIČATKA, M.; KARÁSEK, J.; HEß, C.

Vydáno

19. 9. 2024

Nakladatel

American Society for Microbiology

ISSN

0095-1137

Periodikum

JOURNAL OF CLINICAL MICROBIOLOGY

Číslo

09-2024

Stát

Spojené státy americké

Strany od

1

Strany do

12

Strany počet

12

URL

https://journals.asm.org/doi/10.1128/jcm.00434-24

Plný text v Digitální knihovně

http://hdl.handle.net/11012/249488

BibTex

@article{BUT189446,
  author="Arthur B. {Pranada} and Michal {Čičatka} and Clara {Heß} and Jan {Karásek}",
  title="Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification",
  journal="JOURNAL OF CLINICAL MICROBIOLOGY",
  year="2024",
  number="09-2024",
  pages="12",
  doi="10.1128/jcm.00434-24",
  issn="0095-1137",
  url="https://journals.asm.org/doi/10.1128/jcm.00434-24"
}