Detail publikace

The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering

VERČIMÁKOVÁ, K. KARBOWNICZEK, J. SEDLÁŘ, M. STACHEWICZ, U. VOJTOVÁ, L.

Originální název

The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Various strategies have extensively explored enhancing the physical and biological properties of chitosan and cellulose scaffolds for skin tissue engineering. This study presents a straightforward method involving the addition of glycerol into highly porous structures of two polysaccharide complexes: chitosan/carboxymethyl cellulose (Chit/CMC) and chitosan/oxidized cellulose (Chit/OC); during a one-step freeze-drying process. Adding glycerol, especially to Chit/CMC, significantly increased stability, prevented degradation, and improved mechanical strength by nearly 50%. Importantly, after 21 days of incubation in enzymatic medium Chit/CMC scaffold has almost completely decomposed, while foams reinforced with glycerol exhibited only 40% mass loss. It is possible due to differences in multivalent cations and polymer chain contraction, resulting in varied hydrogen bonding and, consequently, distinct physicochemical outcomes. Additionally, the scaffolds with glycerol improved the cellular activities resulting in over 40% higher proliferation of fibroblast after 21 days of incubation. It was achieved by imparting water resistance to the highly absorbent material and aiding in achieving a balance between hydrophilic and hydrophobic properties. This study clearly indicates the possible elimination of additional crosslinkers and multiple fabrication steps that can reduce the cost of scaffold production for skin tissue engineering applications while tailoring mechanical strength and degradation.

Klíčová slova

Chitosan; Cellulose; Ionic complex; Glycerol; Tissue engineering

Autoři

VERČIMÁKOVÁ, K.; KARBOWNICZEK, J.; SEDLÁŘ, M.; STACHEWICZ, U.; VOJTOVÁ, L.

Vydáno

2. 7. 2024

Nakladatel

ELSEVIER

Místo

AMSTERDAM

ISSN

1879-0003

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

275

Číslo

Volume 275, Part

Stát

Nizozemsko

Strany od

1

Strany do

12

Strany počet

12

URL

https://www.sciencedirect.com/science/article/pii/S0141813024044076#f0005

BibTex

@article{BUT189828,
  author="Katarína {Verčimáková} and Joanna {Karbowniczek} and Marian {Sedlář} and Urszula {Stachewicz} and Lucy {Vojtová}",
  title="The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2024",
  volume="275",
  number="Volume 275, Part",
  pages="1--12",
  doi="10.1016/j.ijbiomac.2024.133602",
  issn="1879-0003",
  url="https://www.sciencedirect.com/science/article/pii/S0141813024044076#f0005"
}