Detail publikace

Detection of antimicrobial resistance genes in chicken feces from metagenomic samples: a comparative study on bioinformatics tools

PARTOVI NASR, M. SCHWARZEROVÁ, J. ČEJKOVÁ, D. ŠABATOVÁ, K. JAKUBÍČKOVÁ, M.

Originální název

Detection of antimicrobial resistance genes in chicken feces from metagenomic samples: a comparative study on bioinformatics tools

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The study "Detection of Antimicrobial Resistance Genes in Chicken Feces from Metagenomic Samples: A Comparative Study on Bioinformatics Tools" by Minoo Partovi Nasr et al. investigates the transmission of antimicrobial resistance genes (ARGs) in chicken feces using metagenomic analysis and bioinformatics tools. DNA from Czech-raised chickens was sequenced via Illumina shotgun metagenomics. Quality control, adaptor trimming, and human genome decontamination were performed before analyzing ARGs with three tools: KARGA, KMA, and AMR-meta, using databases like MEGARes and PanRes. KARGA and KMA showed aligned results, identifying prevalent ARGs such as tet(W) and ant(6) genes, while AMR-meta had limited utility due to low likelihood rates. Relative abundance analysis revealed tetracycline resistance genes as most dominant. This comparative evaluation highlights the strengths of KARGA and KMA in ARG detection for microbiome studies.

Klíčová slova

Bioinformatics, International conference, Scientific meeting, Workshop program

Autoři

PARTOVI NASR, M.; SCHWARZEROVÁ, J.; ČEJKOVÁ, D.; ŠABATOVÁ, K.; JAKUBÍČKOVÁ, M.

Vydáno

30. 9. 2024

Strany počet

1

URL

dechema.converia.de/frontend/index/php

BibTex

@misc{BUT193463,
  author="Minoo {Partovi Nasr} and Jana {Schwarzerová} and Darina {Čejková} and Kateřina {Šabatová} and Markéta {Jakubíčková}",
  title="Detection of antimicrobial resistance genes in chicken feces from metagenomic samples: a comparative study on bioinformatics tools",
  year="2024",
  pages="1",
  url="dechema.converia.de/frontend/index/php",
  note="abstract"
}