Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
VESPALCOVÁ, M. HOLIŠOVÁ, R. VRÁNOVÁ, D. TŮMA, Z.
Originální název
Findings and indication in the PAGE- analysis of sweet protein thaumatin.
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
Thaumatin (E 957) is a low-caloric alternative sweetener produced from fruits of the tropical plant Thaumatococcus danielli Benth.. It is the sweetest known substance, is approximately 3000 times sweeter than sucrose and acts as a taste modifier, too. Chemically, thaumatin is a basic protein, reportedly of extremely high pI = 11.7-12.0 [1] and of relative molecular mass 22 kDa . Commercially available thaumatin preparations consists of two main fractions, thaumatin I and thaumatin II, that differ only by five aminoacids in their chains, and several minor proteinic admixtures. Complete separation of constituents in commercially available thaumatins is very difficult. Well documented analytical technique for the purpose is missing yet. We decided therefore to start systematic research on the thaumatin analysis by exploration on analytical potential of PAGE techniques whose utilization for the purpose is the most frequently reported. Experiments at both non-denaturating and denaturating conditions show that neither thaumatin pI derived from flat bed gel analyses nor efficacy of the SDS-PAGE method for thaumatin, respectively, are reliable. It casts doubt on the capability of flat bed gel analyses to give credible analytical results for thaumatin as the analyte.
Klíčová slova
Thaumatin, PAGE-analysis, SDS-PAGE, basic protein,
Autoři
VESPALCOVÁ, M.; HOLIŠOVÁ, R.; VRÁNOVÁ, D.; TŮMA, Z.
Rok RIV
2005
Vydáno
25. 8. 2005
Nakladatel
Česká společnost chemická
Místo
Brno
ISSN
0009-2770
Periodikum
Chemické listy
Ročník
99
Číslo
S
Stát
Česká republika
Strany od
362
Strany do
364
Strany počet
3
BibTex
@article{BUT42745, author="Milena {Vespalcová} and Radana {Holišová} and Dana {Vránová} and Zdeněk {Tůma}", title="Findings and indication in the PAGE- analysis of sweet protein thaumatin.", journal="Chemické listy", year="2005", volume="99", number="S", pages="362--364", issn="0009-2770" }