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KUBÁŇ, P. PELCOVÁ, P. KUBÁŇ, V. KLAKURKOVÁ, L. PURNENDU K., D.
Originální název
Gravity-flow open tubular cation chromatography
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
We describe ion chromatography (IC) on open tubular cation exchange columns with a controllable capacity multilayered stationary phase architecture. The columns of relatively large bore (75 lm id) are fabricated by coating fused-silica capillaries with multiple layers of poly(butadiene-maleic acid) (PBMA) copolymer and crosslinking the deposited layers by thermally initiated radical polymerisation. Column capacity increases in a predictable manner with increase in the number of successively coated layers. Gravity flow with a modest head (a2 m) can provide the desired separations within a reasonable period. We provide a minimalist configuration where no suppression is used, the sample is injected hydrodynamically as in CE, and detection is accomplished by an inexpensive homebuilt contactless conductivity detector or a capacitance to voltage digital converter. A 1 m long 75 lm bore column coated with two layers of PBMA allows gravity-flow open tubular IC to separate four alkali cations in a10 min with a 1 mM tartaric acid (TA) eluent. Simultaneous separation of alkali and alkaline earth metal cations can be accomplished in less than 25 min using 1.75 mM pyridinedicarboxylic acid as an eluent. Contactless conductometric detection (C4D) allows LODs down to 150 nmol/L, corresponding to 30 fmol injections. Analysis of real water samples is demonstrated.
Klíčová slova
Cations ; Contactless conductometric detection; Gravity-flow; Open tubular ion chromatography
Autoři
KUBÁŇ, P.; PELCOVÁ, P.; KUBÁŇ, V.; KLAKURKOVÁ, L.; PURNENDU K., D.
Rok RIV
2008
Vydáno
20. 8. 2008
Nakladatel
WILEY-VCH Verlag GmbH&Co. KGaA
Místo
Weinheim, DE
ISSN
1615-9306
Periodikum
Journal of Separation Science
Ročník
Číslo
31
Stát
Spolková republika Německo
Strany od
2745
Strany do
2753
Strany počet
9
BibTex
@article{BUT47036, author="Petr {Kubáň} and Pavlína {Pelcová} and Vlastimil {Kubáň} and Lenka {Klakurková} and Dasgupta {Purnendu K.}", title="Gravity-flow open tubular cation chromatography", journal="Journal of Separation Science", year="2008", volume="2008", number="31", pages="2745--2753", issn="1615-9306" }