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STEJSKAL, K. KŘÍŽKOVÁ, S. ADAM, V. SURES, B. TRNKOVÁ, L. ZEHNÁLEK, J. HUBÁLEK, J. BEKLOVÁ, M. HANUSTIAK, P. SVOBODOVÁ, Z. HORNA, A. KIZEK, R.
Originální název
Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique.
Klíčová slova
Amperometric detection; biomarker; electrochemical sensors; fish; flow injection analysis; heavy metal; metallothionein; poecilia reticulata; silver
Autoři
STEJSKAL, K.; KŘÍŽKOVÁ, S.; ADAM, V.; SURES, B.; TRNKOVÁ, L.; ZEHNÁLEK, J.; HUBÁLEK, J.; BEKLOVÁ, M.; HANUSTIAK, P.; SVOBODOVÁ, Z.; HORNA, A.; KIZEK, R.
Rok RIV
2008
Vydáno
1. 10. 2008
Nakladatel
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Místo
USA
ISSN
1530-437X
Periodikum
IEEE SENSORS JOURNAL
Ročník
8
Číslo
9-10
Stát
Spojené státy americké
Strany od
1578
Strany do
1585
Strany počet
BibTex
@article{BUT47213, author="STEJSKAL, K. and KŘÍŽKOVÁ, S. and ADAM, V. and SURES, B. and TRNKOVÁ, L. and ZEHNÁLEK, J. and HUBÁLEK, J. and BEKLOVÁ, M. and HANUSTIAK, P. and SVOBODOVÁ, Z. and HORNA, A. and KIZEK, R.", title="Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection", journal="IEEE SENSORS JOURNAL", year="2008", volume="8", number="9-10", pages="1578--1585", issn="1530-437X" }