Detail publikace

Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

STEJSKAL, K. KŘÍŽKOVÁ, S. ADAM, V. SURES, B. TRNKOVÁ, L. ZEHNÁLEK, J. HUBÁLEK, J. BEKLOVÁ, M. HANUSTIAK, P. SVOBODOVÁ, Z. HORNA, A. KIZEK, R.

Originální název

Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique.

Klíčová slova

Amperometric detection; biomarker; electrochemical sensors; fish; flow injection analysis; heavy metal; metallothionein; poecilia reticulata; silver

Autoři

STEJSKAL, K.; KŘÍŽKOVÁ, S.; ADAM, V.; SURES, B.; TRNKOVÁ, L.; ZEHNÁLEK, J.; HUBÁLEK, J.; BEKLOVÁ, M.; HANUSTIAK, P.; SVOBODOVÁ, Z.; HORNA, A.; KIZEK, R.

Rok RIV

2008

Vydáno

1. 10. 2008

Nakladatel

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

Místo

USA

ISSN

1530-437X

Periodikum

IEEE SENSORS JOURNAL

Ročník

8

Číslo

9-10

Stát

Spojené státy americké

Strany od

1578

Strany do

1585

Strany počet

8

BibTex

@article{BUT47213,
  author="STEJSKAL, K. and KŘÍŽKOVÁ, S. and ADAM, V. and SURES, B. and TRNKOVÁ, L. and ZEHNÁLEK, J. and HUBÁLEK, J. and BEKLOVÁ, M. and HANUSTIAK, P. and SVOBODOVÁ, Z. and HORNA, A. and KIZEK, R.",
  title="Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection",
  journal="IEEE SENSORS JOURNAL",
  year="2008",
  volume="8",
  number="9-10",
  pages="1578--1585",
  issn="1530-437X"
}