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HÚSKA, D. HUBÁLEK, J. ADAM, V. KIZEK, R.
Originální název
Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrodes were placed. Further the amplicons were loaded into agarose gel wells. DNA migrating to the detection place was electrochemically analyzed. Amplicons obtained after two cycles were detectable by this hyphenated technique.
Klíčová slova
Carbon nanotubes; DNA; Gel electrophoresis on chip; PCR; Voltammetry
Klíčová slova v angličtině
Autoři
HÚSKA, D.; HUBÁLEK, J.; ADAM, V.; KIZEK, R.
Rok RIV
2009
Vydáno
1. 1. 2009
Nakladatel
WILEY-V C H VERLAG GMBH
Místo
PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
ISSN
0173-0835
Periodikum
Electrophoresis
Ročník
2009 (29)
Číslo
24
Stát
Spolková republika Německo
Strany od
4964
Strany do
4971
Strany počet
8
BibTex
@article{BUT48742, author="Dalibor {Húska} and Jaromír {Hubálek} and Vojtěch {Adam} and René {Kizek}", title="Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR", journal="Electrophoresis", year="2009", volume="2009 (29)", number="24", pages="4964--4971", issn="0173-0835" }