Detail publikace

Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector

ADAM, V. ZÍTKA, O. DOLEŽAL, P. ZEMAN, L. HORNA, A. HUBÁLEK, J. ŠÍLENÝ, J. KŘÍŽKOVÁ, S. TRNKOVÁ, L. KIZEK, R.

Originální název

Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum samples. Primarily we utilized sodium dodecyl sulphate-polyacrylamide gel electrophoresis for isolation of lactoferrin from the real samples. Moreover we tested automated microfluidic Experion electrophoresis system to isolate lactoferrin from the collostrum sample. The well developed signal of lactoferrin was determined with detection limit (3 S/N) of 20 ng/ml. In spite of the fact that Experion is faster than SDS-PAGE both separation techniques cannot be used in routine analysis. Therefore we have tested third separation technique, ion exchange chromatography, using monolithic column coupled with UV-VIS detector (LCUV-VIS). We optimized wave length (280 nm), ionic strength of the elution solution (1.5 M NaCl) and flow rate of the retention and elution solutions (0.25 ml/min and 0.75 ml/min. respectively).

Klíčová slova

Lactoferrin; Milk Protein; Linear Sweep Voltammetry; Screen-Printed Carbon Electrode; Electrochemistry; Liquid Chromatography; Monolithic Column; UV-VIS Spectrometry

Autoři

ADAM, V.; ZÍTKA, O.; DOLEŽAL, P.; ZEMAN, L.; HORNA, A.; HUBÁLEK, J.; ŠÍLENÝ, J.; KŘÍŽKOVÁ, S.; TRNKOVÁ, L.; KIZEK, R.

Rok RIV

2008

Vydáno

24. 1. 2008

Nakladatel

MDPI

Místo

Basel

ISSN

1424-8220

Periodikum

SENSORS

Ročník

2008

Číslo

8

Stát

Švýcarská konfederace

Strany od

464

Strany do

487

Strany počet

23

URL

http://www.mdpi.com/1424-8220/8/1/464

Plný text v Digitální knihovně

http://hdl.handle.net/11012/70078

BibTex

@article{BUT49158,
  author="ADAM, V. and ZÍTKA, O. and DOLEŽAL, P. and ZEMAN, L. and HORNA, A. and HUBÁLEK, J. and ŠÍLENÝ, J. and KŘÍŽKOVÁ, S. and TRNKOVÁ, L. and KIZEK, R.",
  title="Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector",
  journal="SENSORS",
  year="2008",
  volume="2008",
  number="8",
  pages="464--487",
  doi="10.3390/s8010464",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/8/1/464"
}