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BÉBAROVÁ, M. MATĚJOVIČ, P. PÁSEK, M. ŠIMURDOVÁ, M. NOVÁKOVÁ, M. ŠIMURDA, J.
Originální název
Effect of antipsychotic drug perphenazine on fast sodium current and transient outward potassium current in rat ventricular myocytes
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
Antipsychotic drug perphenazine belongs to the phenothiazine group commonly reported to induce ECG changes and tachyarrhythmias. Data about its effect on ionic membrane currents in cardiomyocytes are missing. We analyzed the effect of perphenazine (0.1-100 uM) on fast sodium current INa and transient outward potassium current Ito in enzymatically isolated rat right ventricular myocytes by the whole-cell patch-clamp technique at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50=1.24+-0.10 uM) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50=38.2+-3.5 uM, evaluated from changes of the time integral). The fast time constant of Ito inactivation was significantly decreased in a concentration-dependent manner (IC50=30.0+-6.6 uM). Both blocks were use and frequency dependent at 3.3 Hz. We conclude that perphenazine causes concentration-, use-, and frequency-dependent block of INa
Klíčová slova
Perphenazine; Sodium current; Transient outward potasium current; Mathematical simulations; Ventricular myocytes
Autoři
BÉBAROVÁ, M.; MATĚJOVIČ, P.; PÁSEK, M.; ŠIMURDOVÁ, M.; NOVÁKOVÁ, M.; ŠIMURDA, J.
Rok RIV
2009
Vydáno
9. 5. 2009
Nakladatel
Springer -Verlag
ISSN
0028-1298
Periodikum
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Ročník
380
Číslo
5
Stát
Spolková republika Německo
Strany od
125
Strany do
133
Strany počet
9
BibTex
@article{BUT49584, author="Markéta {Bébarová} and Peter {Matějovič} and Michal {Pásek} and Milena {Šimurdová} and Marie {Nováková} and Jiří {Šimurda}", title="Effect of antipsychotic drug perphenazine on fast sodium current and transient outward potassium current in rat ventricular myocytes", journal="NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY", year="2009", volume="380", number="5", pages="125--133", issn="0028-1298" }