Detail publikace

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

RITTICH, B. ŠPANOVÁ, A. TRACHTOVÁ, Š. HORÁK, D.

Originální název

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.

Klíčová slova

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR

Autoři

RITTICH, B.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.

Vydáno

30. 6. 2010

Nakladatel

VUTIUM

Místo

Brno

ISBN

978-80-214-4112-5

Kniha

Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.

Strany od

151

Strany do

151

Strany počet

1

BibTex

@misc{BUT72665,
  author="RITTICH, B. and ŠPANOVÁ, A. and TRACHTOVÁ, Š. and HORÁK, D.",
  title="Magnetic Microspheres for DNA Diagnostics and DNA Delivery",
  booktitle="Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.",
  year="2010",
  pages="151--151",
  publisher="VUTIUM",
  address="Brno",
  isbn="978-80-214-4112-5",
  note="abstract"
}