Detail publikace

Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation

TRACHTOVÁ, Š. KAMAN, O. ŠPANOVÁ, A. VEVERKA, P. POLLERT, E. RITTICH, B.

Originální název

Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20 nm thick silica shell (LSMO(0.25)SiO2) were characterised and tested for the isolation of PCR ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16 % PEG 6000, 2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.

Klíčová slova

nanoparticles; lanthanum manganese perovskites; silica shell; DNA isolation; PCR

Autoři

TRACHTOVÁ, Š.; KAMAN, O.; ŠPANOVÁ, A.; VEVERKA, P.; POLLERT, E.; RITTICH, B.

Rok RIV

2011

Vydáno

1. 11. 2011

Nakladatel

John Wiley and Sons

Místo

WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany

ISSN

1615-9306

Periodikum

Journal of Separation Science

Ročník

34

Číslo

21

Stát

Spolková republika Německo

Strany od

3077

Strany do

3082

Strany počet

6

URL

http://onlinelibrary.wiley.com.ezproxy.lib.vutbr.cz/doi/10.1002/jssc.201100442/epdf

BibTex

@article{BUT73164,
  author="TRACHTOVÁ, Š. and KAMAN, O. and ŠPANOVÁ, A. and VEVERKA, P. and POLLERT, E. and RITTICH, B.",
  title="Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation",
  journal="Journal of Separation Science",
  year="2011",
  volume="34",
  number="21",
  pages="3077--3082",
  doi="10.1002/jssc.201100442",
  issn="1615-9306",
  url="http://onlinelibrary.wiley.com.ezproxy.lib.vutbr.cz/doi/10.1002/jssc.201100442/epdf"
}