Detail publikace

Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces

TRACHTOVA, S. OBERMAJER, T. SPANOVA, A. BOGOVIC MATIJASIC, B. ROGELJ, I. HORAK, D. RITTICH, B.

Originální název

Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxyl-coated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.

Klíčová slova

magnetic microspheres; c); DNA isolation; mouse faeces; polymerase chain reaction (PCR)

Autoři

TRACHTOVA, S.; OBERMAJER, T.; SPANOVA, A.; BOGOVIC MATIJASIC, B.; ROGELJ, I.; HORAK, D.; RITTICH, B.

Rok RIV

2012

Vydáno

15. 2. 2012

Nakladatel

Taylor & Francis

Místo

Londýn, Spojené království Velké Británie a Severního Irska

ISSN

1542-1406

Periodikum

MOLECULAR CRYSTALS AND LIQUID CRYSTALS

Ročník

555

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

263

Strany do

270

Strany počet

7

BibTex

@article{BUT73651,
  author="Štěpánka {Trachtová} and Tanja {Obermajer} and Alena {Španová} and Bojana {Bogovič Matijašić} and Irena {Rogelj} and Daniel {Horák} and Bohuslav {Rittich}",
  title="Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces",
  journal="MOLECULAR CRYSTALS AND LIQUID CRYSTALS",
  year="2012",
  volume="555",
  number="1",
  pages="263--270",
  doi="10.1080/15421406.2012.635554",
  issn="1542-1406"
}