Detail publikace

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

ZÍTKA, O. HUBÁLEK, J. KŘÍŽKOVÁ, S. HÚSKA, D. ADAM, V. ECKSCHLAGER, T. KIZEK, R.

Originální název

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Matrix metalloproteinases (MMPs) play numerous roles in physiological and pathological processes including cancer. Interactions of MMPs with other zinc-binding proteins are of great interest mainly from the point of view of the participation of these proteins in carcinogenesis. The aim of this study was to utilize chip gel electrophoresis for investigation of matrix metalloproteinase 9 (gelatinase B, MMP-9) interactions with collagen. The interaction was observed and the effect of temperature was tested. We further focused on the study of activation of MMP-9 by the presence of zinc-binding proteins called metallothioneins (MT). We confirmed the basic presumption of the activation of MMP-9 by the presence of MT similar to the temperature effect. Moreover, we chose the method of differential pulse voltammetry Brdicka reaction to verify our results. It can be concluded that the activity of MMP-9 was higher in the presence of MT in comparison with the experimental collagen(type I)-MMP-9 mixture in vitro.

Klíčová slova

matrix metalloproteinases, carcinogenesis, cancer

Autoři

ZÍTKA, O.; HUBÁLEK, J.; KŘÍŽKOVÁ, S.; HÚSKA, D.; ADAM, V.; ECKSCHLAGER, T.; KIZEK, R.

Rok RIV

2011

Vydáno

1. 9. 2011

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

2011 (32)

Číslo

8

Stát

Spolková republika Německo

Strany od

857

Strany do

860

Strany počet

4

BibTex

@article{BUT74828,
  author="ZÍTKA, O. and HUBÁLEK, J. and KŘÍŽKOVÁ, S. and HÚSKA, D. and ADAM, V. and ECKSCHLAGER, T. and KIZEK, R.",
  title="Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein",
  journal="Electrophoresis",
  year="2011",
  volume="2011 (32)",
  number="8",
  pages="857--860",
  issn="0173-0835"
}