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OHNOUTKOVÁ, L. ZÍTKA, O. MRÍZOVÁ, K. VALÁŠKOVÁ, J. GALUSZKA, P. CERNEI, N. SMEDLEY, M. HARWOOD, W. ADAM, V. KIZEK, R.
Originální název
Electrophoretic and chromatographic evaluation of transgenic Barley expressing a bacterial dihydrodipicolinate synthase
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Nutritional quality of human and animal foodstuffs is determined by the content of essential amino acids. Barley is the fourth most important cereal of the world and the second most important cereal grown in the Czech Republic. Cereal grains such as barley contain insufficient levels of some essential amino acids, especially lysine. Dihydrodipicolinate synthase is the key enzyme involved in the regulatory step for lysine biosynthesis. Two constructs pBract214::sTPdapA and pBract214::mdapA containing the dapA gene from Escherichia coli coding for the bacterial dihydrodipicolinate synthase were used for transformation of barley. An Agrobacterium-mediated technique was used for transformation of immature embryos of spring barley cv. Golden Promise. Transgenic barley plants of the T0 and T1 generations were evaluated by PCR, real-time PCR, gel electrophoresis, and Western blot. Amino acid content was analyzed by HPLC after HCl hydrolysis. The lysine content in leaves of the T1 generation plant no. 5/5 was 50% higher than in wild-type plants; the lysine content in seeds of T2 generation plant no. 5/16 was 30% higher than in wild-type seeds of spring barley cv. Golden Promise.
Klíčová slova
Foodomics, Gel electrophoresis, Polymerase chain reaction, Transgenic barley, Western blot
Autoři
OHNOUTKOVÁ, L.; ZÍTKA, O.; MRÍZOVÁ, K.; VALÁŠKOVÁ, J.; GALUSZKA, P.; CERNEI, N.; SMEDLEY, M.; HARWOOD, W.; ADAM, V.; KIZEK, R.
Rok RIV
2012
Vydáno
1. 8. 2012
ISSN
0173-0835
Periodikum
Electrophoresis
Ročník
33
Číslo
15
Stát
Spolková republika Německo
Strany od
2365
Strany do
2373
Strany počet
9
BibTex
@article{BUT92847, author="Ludmila {Ohnoutková} and Ondřej {Zítka} and Katarína {Mrízová} and Jana {Valášková} and Petr {Galuszka} and Natalia Vladimirovna {Cernei} and Mark A. {Smedley} and Wendy A. {Harwood} and Vojtěch {Adam} and René {Kizek}", title="Electrophoretic and chromatographic evaluation of transgenic Barley expressing a bacterial dihydrodipicolinate synthase", journal="Electrophoresis", year="2012", volume="33", number="15", pages="2365--2373", doi="10.1002/elps.201200033", issn="0173-0835" }