Detail publikace

Electrophoretic and chromatographic evaluation of transgenic Barley expressing a bacterial dihydrodipicolinate synthase

OHNOUTKOVÁ, L. ZÍTKA, O. MRÍZOVÁ, K. VALÁŠKOVÁ, J. GALUSZKA, P. CERNEI, N. SMEDLEY, M. HARWOOD, W. ADAM, V. KIZEK, R.

Originální název

Electrophoretic and chromatographic evaluation of transgenic Barley expressing a bacterial dihydrodipicolinate synthase

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Nutritional quality of human and animal foodstuffs is determined by the content of essential amino acids. Barley is the fourth most important cereal of the world and the second most important cereal grown in the Czech Republic. Cereal grains such as barley contain insufficient levels of some essential amino acids, especially lysine. Dihydrodipicolinate synthase is the key enzyme involved in the regulatory step for lysine biosynthesis. Two constructs pBract214::sTPdapA and pBract214::mdapA containing the dapA gene from Escherichia coli coding for the bacterial dihydrodipicolinate synthase were used for transformation of barley. An Agrobacterium-mediated technique was used for transformation of immature embryos of spring barley cv. Golden Promise. Transgenic barley plants of the T0 and T1 generations were evaluated by PCR, real-time PCR, gel electrophoresis, and Western blot. Amino acid content was analyzed by HPLC after HCl hydrolysis. The lysine content in leaves of the T1 generation plant no. 5/5 was 50% higher than in wild-type plants; the lysine content in seeds of T2 generation plant no. 5/16 was 30% higher than in wild-type seeds of spring barley cv. Golden Promise.

Klíčová slova

Foodomics, Gel electrophoresis, Polymerase chain reaction, Transgenic barley, Western blot

Autoři

OHNOUTKOVÁ, L.; ZÍTKA, O.; MRÍZOVÁ, K.; VALÁŠKOVÁ, J.; GALUSZKA, P.; CERNEI, N.; SMEDLEY, M.; HARWOOD, W.; ADAM, V.; KIZEK, R.

Rok RIV

2012

Vydáno

1. 8. 2012

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

33

Číslo

15

Stát

Spolková republika Německo

Strany od

2365

Strany do

2373

Strany počet

9

BibTex

@article{BUT92847,
  author="Ludmila {Ohnoutková} and Ondřej {Zítka} and Katarína {Mrízová} and Jana {Valášková} and Petr {Galuszka} and Natalia Vladimirovna {Cernei} and Mark A. {Smedley} and Wendy A. {Harwood} and Vojtěch {Adam} and René {Kizek}",
  title="Electrophoretic and chromatographic evaluation of transgenic Barley expressing a bacterial dihydrodipicolinate synthase",
  journal="Electrophoresis",
  year="2012",
  volume="33",
  number="15",
  pages="2365--2373",
  doi="10.1002/elps.201200033",
  issn="0173-0835"
}