Detail publikace

Study of DNA–ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection

RYVOLOVÁ, M. ADAM, V. ECKSCHLAGER, T. STIBOROVÁ, M. KIZEK, R.

Originální název

Study of DNA–ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence (CE-LIF) detection has been developed for the determination of ellipticine and for the monitoring of ellipticine–DNA interaction. Sodium acetate (50 mM, pH 4.5) was used as a background electrolyte and LIF detection at ex = 488 nm. The limit of detection for ellipticine was determined to be 5 10 8 M. A total of 20% dimethyl sulfoxide was found optimal as sample solvent. Additionally, intercalation of ellipticine into the double-stranded DNA was investigated. Signal corresponding to ellipticine was decreasing and a new peak appeared and was growing. It can be concluded that CE-LIF is a method applicable to in vitro studies of ellipticine–DNA complexes.

Klíčová slova

Capillary electrophoresis; DNA; Ellipticine;Tumor disease

Autoři

RYVOLOVÁ, M.; ADAM, V.; ECKSCHLAGER, T.; STIBOROVÁ, M.; KIZEK, R.

Rok RIV

2012

Vydáno

27. 6. 2012

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

33

Číslo

11

Stát

Spolková republika Německo

Strany od

1545

Strany do

1549

Strany počet

5

BibTex

@article{BUT92848,
  author="Markéta {Vaculovičová} and Vojtěch {Adam} and Tomáš {Eckschlager} and Marie {Stiborová} and René {Kizek}",
  title="Study of DNA–ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection",
  journal="Electrophoresis",
  year="2012",
  volume="33",
  number="11",
  pages="1545--1549",
  doi="10.1002/elps.201200056",
  issn="0173-0835"
}