Detail publikace

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

CASTILLO, G. TRNKOVÁ, L. HRDÝ, R. HIANIK, T.

Originální název

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3/4. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mM with a limit of detection of 5 nM

Klíčová slova

DNA aptamers, Biosensors, Human thrombin, Electrochemical impedance spectroscopy

Autoři

CASTILLO, G.; TRNKOVÁ, L.; HRDÝ, R.; HIANIK, T.

Rok RIV

2012

Vydáno

1. 5. 2012

ISSN

1040-0397

Periodikum

ELECTROANALYSIS

Ročník

24

Číslo

5

Stát

Spolková republika Německo

Strany od

1079

Strany do

1087

Strany počet

9

BibTex

@article{BUT92982,
  author="Gabriela {Castillo} and Libuše {Trnková} and Radim {Hrdý} and Tibor {Hianik}",
  title="Impedimetric Aptasensor for Thrombin Recognition Based on CD Support",
  journal="ELECTROANALYSIS",
  year="2012",
  volume="24",
  number="5",
  pages="1079--1087",
  doi="10.1002/elan.201200041",
  issn="1040-0397"
}