Detail publikace

Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining

MASAŘÍK, M. GUMULEC, J. HLAVNA, M. SZTALMACHOVÁ, M. BABULA, P. RAUDENSKÁ, M. PÁVKOVÁ GOLDBERGOVÁ, M. CERNEI, N. SOCHOR, J. ZÍTKA, O. RUTTKAY-NEDECKÝ, B. KŘÍŽKOVÁ, S. ADAM, V. KIZEK, R.

Originální název

Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The present paper is focused on zinc(II) treatment effects on prostatic cell lines PC-3 (tumour) and PNT1A (non-tumour). Oxidative status of cells was monitored by evaluation of expression of metallothionein (MT) isoforms 1A and 2A at the mRNA and protein level, glutathione (oxidised and reduced), and intracellular zinc(II) after exposition to zinc(II) treatment at concentrations of 0–150 mM using electrochemical methods, western blotting and fluorescent microscopy. When real-time growth monitoring, precise electrochemical methods and fluorescent microscopy are performed together, accurate information for metal fluxes, their buffering by thiol compounds and monitoring of the redox state become a powerful tool for understanding the role of oxidative stress in carcinogenesis

Klíčová slova

OXIDATIVE STRESS; CANCER CELLS; LIQUID-CHROMATOGRAPHY; CARCINOMA-CELLS; ACRIDINE-ORANGE; ZINC; METALLOTHIONEIN; DIFFERENTIATION; TRANSPORTER; GLUTATHIONE

Autoři

MASAŘÍK, M.; GUMULEC, J.; HLAVNA, M.; SZTALMACHOVÁ, M.; BABULA, P.; RAUDENSKÁ, M.; PÁVKOVÁ GOLDBERGOVÁ, M.; CERNEI, N.; SOCHOR, J.; ZÍTKA, O.; RUTTKAY-NEDECKÝ, B.; KŘÍŽKOVÁ, S.; ADAM, V.; KIZEK, R.

Rok RIV

2012

Vydáno

13. 4. 2012

ISSN

1757-9694

Periodikum

Integrative Biology

Ročník

6

Číslo

4

Stát

Spojené království Velké Británie a Severního Irska

Strany od

672

Strany do

684

Strany počet

13

BibTex

@article{BUT92997,
  author="Michal {Masařík} and Jaromír {Gumulec} and Marian {Hlavna} and Markéta {Sztalmachová} and Petr {Babula} and Martina {Raudenská} and Monika {Pávková Goldbergová} and Natalia Vladimirovna {Cernei} and Jiří {Sochor} and Ondřej {Zítka} and Branislav {Ruttkay-Nedecký} and Soňa {Křížková} and Vojtěch {Adam} and René {Kizek}",
  title="Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining",
  journal="Integrative Biology",
  year="2012",
  volume="6",
  number="4",
  pages="672--684",
  doi="10.1039/c2ib00157h",
  issn="1757-9694"
}