Detail publikace

Effect of zinc(II) ions on the expression of pro- and anti-apoptotic factors in high-grade prostate carcinoma cells

SZTALMACHOVÁ, M. HLAVNA, M. GUMULEC, J. HOLUBOVÁ, M. BABULA, P. BALVAN, J. SOCHOR, J. TANHÄUSEROVÁ, V. RAUDENSKÁ, M. KŘÍŽKOVÁ, S. ADAM, V. ECKSCHLAGER, T. KIZEK, R. MASAŘÍK, M.

Originální název

Effect of zinc(II) ions on the expression of pro- and anti-apoptotic factors in high-grade prostate carcinoma cells

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy

Klíčová slova

apoptosis, prostate cancer, prognostic marker, Bcl-2, p53, polymerase chain reaction, thiol group, fluorescence microscopy

Autoři

SZTALMACHOVÁ, M.; HLAVNA, M.; GUMULEC, J.; HOLUBOVÁ, M.; BABULA, P.; BALVAN, J.; SOCHOR, J.; TANHÄUSEROVÁ, V.; RAUDENSKÁ, M.; KŘÍŽKOVÁ, S.; ADAM, V.; ECKSCHLAGER, T.; KIZEK, R.; MASAŘÍK, M.

Rok RIV

2012

Vydáno

1. 9. 2012

ISSN

1021-335X

Periodikum

ONCOLOGY REPORTS

Ročník

28

Číslo

3

Stát

Řecká republika

Strany od

806

Strany do

814

Strany počet

9

BibTex

@article{BUT93015,
  author="Markéta {Sztalmachová} and Marian {Hlavna} and Jaromír {Gumulec} and Monika {Holubová} and Petr {Babula} and Jan {Balvan} and Jiří {Sochor} and Veronika {Tanhäuserová} and Martina {Raudenská} and Soňa {Křížková} and Vojtěch {Adam} and Tomáš {Eckschlager} and René {Kizek} and Michal {Masařík}",
  title="Effect of zinc(II) ions on the expression of pro- and anti-apoptotic factors in high-grade prostate carcinoma cells",
  journal="ONCOLOGY REPORTS",
  year="2012",
  volume="28",
  number="3",
  pages="806--814",
  issn="1021-335X"
}