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PEKAROVÁ, M. KUCHTA, R. KADLEC, J.
Originální název
The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
It is known that cells and organisms can indirectly sense changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cells have not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxide synthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, l-arginine was shown to modulate the response of macrophages toward lipopolysaccharide via the activation of G-protein-coupled receptors. According to our data, we concluded that l-arginine availability plays a key role in the initiation of intracellular signaling pathways that trigger the lipopolysaccharide-induced inflammatory responses in murine macrophages. Although macrophages are partially stimulated in the absence of extracellular l-arginine, the presence of this amino acid significantly accelerates the sensitivity of macrophages to bacterial endotoxin.
Klíčová slova
Macrophages; l-arginine; Lipopolysaccharide; Mitogen-activated protein kinases; Phospholipase C; G-protein-coupled receptor
Autoři
PEKAROVÁ, M.; KUCHTA, R.; KADLEC, J.
Rok RIV
2013
Vydáno
27. 11. 2012
Nakladatel
PublisherSpringer-Verlag
ISSN
0257-277X
Periodikum
IMMUNOLOGIC RESEARCH
Ročník
2012
Číslo
11
Stát
Spojené státy americké
Strany od
2443
Strany do
2457
Strany počet
12
BibTex
@article{BUT95688, author="Michaela {Pekarová} and Radek {Kuchta} and Jaroslav {Kadlec} and Zdeňka {Kuchtová}", title="The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin", journal="IMMUNOLOGIC RESEARCH", year="2012", volume="2012", number="11", pages="2443--2457", issn="0257-277X" }