Detail publikace

The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

PEKAROVÁ, M. KUCHTA, R. KADLEC, J.

Originální název

The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

It is known that cells and organisms can indirectly sense changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cells have not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxide synthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, l-arginine was shown to modulate the response of macrophages toward lipopolysaccharide via the activation of G-protein-coupled receptors. According to our data, we concluded that l-arginine availability plays a key role in the initiation of intracellular signaling pathways that trigger the lipopolysaccharide-induced inflammatory responses in murine macrophages. Although macrophages are partially stimulated in the absence of extracellular l-arginine, the presence of this amino acid significantly accelerates the sensitivity of macrophages to bacterial endotoxin.

Klíčová slova

Macrophages; l-arginine; Lipopolysaccharide; Mitogen-activated protein kinases; Phospholipase C; G-protein-coupled receptor

Autoři

PEKAROVÁ, M.; KUCHTA, R.; KADLEC, J.

Rok RIV

2013

Vydáno

27. 11. 2012

Nakladatel

PublisherSpringer-Verlag

ISSN

0257-277X

Periodikum

IMMUNOLOGIC RESEARCH

Ročník

2012

Číslo

11

Stát

Spojené státy americké

Strany od

2443

Strany do

2457

Strany počet

12

BibTex

@article{BUT95688,
  author="Michaela {Pekarová} and Radek {Kuchta} and Jaroslav {Kadlec} and Zdeňka {Kuchtová}",
  title="The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin",
  journal="IMMUNOLOGIC RESEARCH",
  year="2012",
  volume="2012",
  number="11",
  pages="2443--2457",
  issn="0257-277X"
}