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ZÍTKA, O. KOMÍNKOVÁ, M. SKALIČKOVÁ, S. ŠKUTKOVÁ, H. PROVAZNÍK, I. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. TRNKOVÁ, L. KIZEK, R.
Originální název
Hydrodynamic Voltammograms Profiling of Metallothionein Fragment
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Metallothionein is intracellular metal binding protein. It can bind essential zinc ions and also toxic metals like cadmium. In cancer research the problem of resistance of cancer cells against anticancer drugs was described. In this study we optimized an electrochemical method for exploring the differences in electrochemical signals in dependence on amino acid sequence in metallothionein. For this purpose we selected the number of fragments based on bioinformatic processing, synthesized them and made high throughput electrochemical analysis. Designed electrochemical analysis was based on automated flow injection analysis with amperometric detector using Coulochem III. Detection was carried out by glassy carbon electrode. We measured hydrodynamic voltammograms (HDVs) for 23 selected metallothionein fragments within the potential range from 100 to 1200 mV. We estimated the maxima of oxidation by processing of cumulative HDV. The oxidation maxima were dependent on amino acid composition, whereas fragments from human isoforms and some saltwater fishes species had the highest maxima (900 mV). On the opposite side fragments of other mammals like horse, mouse and bull had the lowest potential maxima (700 mV). This should be related with the binding capacity for metal ions.
Klíčová slova
Metallothionein; Peptides; Flow Injection Analysis; High Throughput Analysis; Bioinformatics; Bioanalysis, Electrochemistry, Carbon Electrode; Sulfhydryl Group
Autoři
ZÍTKA, O.; KOMÍNKOVÁ, M.; SKALIČKOVÁ, S.; ŠKUTKOVÁ, H.; PROVAZNÍK, I.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; TRNKOVÁ, L.; KIZEK, R.
Rok RIV
2012
Vydáno
1. 11. 2012
ISSN
1452-3981
Periodikum
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Ročník
7
Číslo
11
Stát
Srbská republika
Strany od
10544
Strany do
10561
Strany počet
18
BibTex
@article{BUT96342, author="Ondřej {Zítka} and Markéta {Komínková} and Sylvie {Skaličková} and Helena {Vítková} and Valentine {Provazník} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and Libuše {Trnková} and René {Kizek}", title="Hydrodynamic Voltammograms Profiling of Metallothionein Fragment", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2012", volume="7", number="11", pages="10544--10561", issn="1452-3981" }