Detail publikace

Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes

DVOŘÁK, M. GREGUŠOVÁ, B. ŠURANSKÁ, H. TURKOVÁ, K. POŘÍZKA, J. VESPALCOVÁ, M. RITTICH, B.

Originální název

Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Determination of organic acids is an important control steps in many biotechnological processes – especially during microbial cultivation, must fermentation. Moreover, in many biotechnological products the final profile of the organic acids is an important factor. Determination of organic acids requires a fast and sensitive analytical method. Capillary zone electrophoresis (CZE) is a very suitable method for the determination of short-chain carboxylic acids, because they are easy to ionize. Further, CZE is an ecological and economical method, have low exigencies to quantity of samples. Method is fast, accurate and sensitive to low concentrations of analytes. Because short-chain aliphatic acids have not suitable chromophore, it is necessary to use indirect photometric detection for their determination. It was tested and optimized background electrolyte containing 3,5-dinitrobenzoic acid (10 – 20 mM) as visualizing and buffering ion, set up to the pH 3,5 – 4,0 (NaOH, TRIS). Cationic surfactants CTAB and TTAB as modifiers of the electroosmotic flow were tested. Separation voltage was set up at +30 kV (inverted polarity), detection indirect UV at 254 nm. Uncoated fused silica capillary was used, total length 75 cm, I.D. 50 um. Two different effective lengths were tested (25 and 50 cm). System was operated at laboratory temperature. Basic organic acids (lactic, malic, tartaric, acetic, succinic, citric, propionic, butyric, valeric, formic) were separated and analyzed in the real matrices (grape must, cultivation media, wine). Results of the determination selected organic acids in the real samples were compared with results obtained from the gas chromatography method. Optimized method using capillary zone electrophoresis with indirect photometry detection is in comparison with chromatography techniques faster and more economical and less sensitive (but sufficient) method.

Klíčová slova

organic acids, capillary zone electrophoresis (CZE), analytical methods

Autoři

DVOŘÁK, M.; GREGUŠOVÁ, B.; ŠURANSKÁ, H.; TURKOVÁ, K.; POŘÍZKA, J.; VESPALCOVÁ, M.; RITTICH, B.

Rok RIV

2012

Vydáno

7. 12. 2012

Nakladatel

Vysoké učení technické v Brně, Fakulta chemická,

Místo

Brno

ISBN

978-80-214-4644-1

Kniha

Studentská odborná konference Chemie je život 2012 - Sborník příspěvků.

Číslo edice

1

Strany od

271

Strany do

275

Strany počet

5

BibTex

@inproceedings{BUT97221,
  author="Miloš {Dvořák} and Barbora {Gregušová} and Hana {Šuranská} and Kristýna {Turková} and Jaromír {Pořízka} and Milena {Vespalcová} and Bohuslav {Rittich}",
  title="Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes",
  booktitle="Studentská odborná konference Chemie je život 2012 - Sborník příspěvků.",
  year="2012",
  number="1",
  pages="271--275",
  publisher="Vysoké učení technické v Brně, Fakulta chemická,",
  address="Brno",
  isbn="978-80-214-4644-1"
}