Detail publikace

In vitro motor patterns and electrophysiological changes in patients with colonic diverticular disease

MIKULKA, J. ŠMIRG, O. GALLEGO, D. ESPÍN, F. GIL, V. FAÚNDEZ ZANUY, M. JIMÉNEZ, M. CLAVÉ, P.

Originální název

In vitro motor patterns and electrophysiological changes in patients with colonic diverticular disease

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

PURPOSE: The underlying mechanism responsible for motility changes in colonic diverticular disease (DD) is still unknown. In the present study, our aim was to investigate the structural and in vitro motor changes in patients with DD. METHOD: Muscle bath, microelectrodes and immunohistochemical techniques were performed with samples obtained from the left and sigmoid colon of patients with DD and compared with those of patients without DD. RESULTS: The amplitude and area under the curve of the spontaneous rhythmic phasic contractions (SRPC) were greatly reduced in patients with DD whereas their frequency and tone remained unaltered. Electrical field stimulation induced a neurallymediated, enhanced ON-contraction (amplitude) in patients with DD and increased the duration of latency of OFF-contractions. The resting membrane potential of smooth muscle cells was hyperpolarized and the amplitude of the inhibitory junction potential (IJP) was increased in patients with DD. In contrast, no significant histological differences were observed in patients with DD as smooth muscle (circular and longitudinal layers), ICC, glial cells and myenteric neurons densities remained unaltered. CONCLUSION: Sigmoid strips from patients with asymptomatic DD showed an altered motor pattern with impaired, neurally-mediated colonic responses involving both excitatory and inhibitory motor pathways. No major neural and muscular structural elements were detected at this stage of the disease. These findings could be valuable in understanding the pathophysiology of this prevalent digestive disease.

Klíčová slova

diverticular disease, in vitro motor patterns changes, electrophysiological changes

Autoři

MIKULKA, J.; ŠMIRG, O.; GALLEGO, D.; ESPÍN, F.; GIL, V.; FAÚNDEZ ZANUY, M.; JIMÉNEZ, M.; CLAVÉ, P.

Rok RIV

2013

Vydáno

24. 5. 2013

ISSN

0179-1958

Periodikum

INTERNATIONAL JOURNAL OF COLORECTAL DISEASE

Ročník

2013

Číslo

5

Stát

Spolková republika Německo

Strany od

1

Strany do

34

Strany počet

34

BibTex

@article{BUT99540,
  author="Jan {Mikulka} and Ondřej {Šmirg} and Diana {Gallego} and Francisco {Espín} and Víctor {Gil} and Marcos {Faúndez Zanuy} and Marcel {Jiménez} and Pere {Clavé}",
  title="In vitro motor patterns and electrophysiological changes in patients with colonic diverticular disease",
  journal="INTERNATIONAL JOURNAL OF COLORECTAL DISEASE",
  year="2013",
  volume="2013",
  number="5",
  pages="1--34",
  issn="0179-1958"
}